Keller J T, Bubel H C, Wander A H, Tierney B E
Christ Hospital, Department of Neurosurgery, Cincinnati, OH.
Neurosci Lett. 1991 Jan 2;121(1-2):247-50. doi: 10.1016/0304-3940(91)90696-q.
The size and somatotopic distribution of corneal afferent neurons in the guinea pig trigeminal ganglion were determined using a retrograde axonal tracing technique. Wheat germ agglutinin-horseradish peroxidase (WGA-HRP) was applied to the central cornea of the guinea pig and the animals were perfusion-fixed 48 h later. In addition, a preliminary study examined corneal afferent neurons in two animals latently infected with the herpes simplex virus by corneal inoculation. The majority of WGA-HRP-labelled neurons were located in the ophthalmic division of the ipsilateral ganglion. A clear dorsoventral somatotopic arrangement of labelled corneal afferent neurons was noted. The size of the neurons averaged 23 microns and the number of cells per ganglion averaged 205. By contrast, the number of labelled neurons in latently infected ganglia averaged less than 50. No size or morphological distinctions could be made between neurons from uninfected or latently infected ganglia. The results of this study have provided for the first time the precise location and somata diameter of primary afferent corneal neurons within the guinea pig trigeminal ganglion.
采用逆行轴突示踪技术确定豚鼠三叉神经节中角膜传入神经元的大小和躯体定位分布。将小麦胚凝集素-辣根过氧化物酶（WGA-HRP）应用于豚鼠的中央角膜，48小时后对动物进行灌注固定。此外，一项初步研究检查了两只通过角膜接种潜伏感染单纯疱疹病毒的动物的角膜传入神经元。大多数WGA-HRP标记的神经元位于同侧神经节的眼支。观察到标记的角膜传入神经元有明显的背腹躯体定位排列。神经元大小平均为23微米，每个神经节的细胞数量平均为205个。相比之下，潜伏感染神经节中标记神经元的数量平均少于50个。未感染或潜伏感染神经节的神经元在大小或形态上没有差异。本研究结果首次提供了豚鼠三叉神经节内初级传入角膜神经元的精确位置和胞体直径。
