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[生物膜的结构:利用特异性抗体对磺基喹喔啉二酰甘油在叶绿体各膜中的定位]

[The structure of biological membranes: localization of sulfoquinovosyldiglyceride in various membranes of chloroplasts using specific antibodies].

作者信息

Billecocq A

出版信息

Ann Immunol (Paris). 1975 Apr;126(3):337-52.

PMID:170851
Abstract

The sulfoquinovosyl pole of at least a part of the sulfolipid molecule, in the envelope and the thylakoids of chloroplasts, is accessible to the specific antibody. Agglutination of the chloroplasts is due to the sulfolipid which is located on the envelope and uniformly distributed on the external surface. Reactivity of the sulfolipid located on the membrane of thylakoids, when exposed to the corresponding antibody, appears to be different from the sulfolipid reactivity of the envelope: the antibody fails to agglutinate the thylakoids. Thylakoids altered by osmotic chock are sensitized to agglutination. Immunoenzymatic studies followed by electron microscopy revealed the presence of the sulfolipid on the membrane of the various thylakoids. The local reactions did not appear to be continous.

摘要

在叶绿体的包膜和类囊体中,至少部分硫脂分子的磺基喹喔啉糖基极可被特异性抗体识别。叶绿体的凝集是由于位于包膜上并均匀分布于外表面的硫脂。当类囊体膜上的硫脂暴露于相应抗体时,其反应性似乎与包膜硫脂的反应性不同:该抗体无法使类囊体凝集。经渗透休克改变的类囊体对凝集敏感。免疫酶学研究及随后的电子显微镜检查揭示了各种类囊体膜上存在硫脂。局部反应似乎并不连续。

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[The structure of biological membranes: localization of sulfoquinovosyldiglyceride in various membranes of chloroplasts using specific antibodies].[生物膜的结构:利用特异性抗体对磺基喹喔啉二酰甘油在叶绿体各膜中的定位]
Ann Immunol (Paris). 1975 Apr;126(3):337-52.
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Localization of the tri- and digalactosyl diglyceride in the thylakoid membrane with serological methods.用血清学方法对三半乳糖基二甘油酯和二半乳糖基二甘油酯在类囊体膜中的定位研究
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引用本文的文献

1
Chloroplast envelope membranes: a dynamic interface between plastids and the cytosol.叶绿体包膜膜:质体与细胞质之间的动态界面。
Photosynth Res. 2007 May;92(2):225-44. doi: 10.1007/s11120-007-9195-8. Epub 2007 Jun 9.
2
Strong binding of cytochrome C on the envelope of spinach chloroplasts.细胞色素 C 与菠菜叶绿体包膜的强结合。
Plant Physiol. 1977 Jun;59(6):1178-81. doi: 10.1104/pp.59.6.1178.
3
Protein translocation into and across the chloroplastic envelope membranes.蛋白质转运进入并穿过叶绿体包膜膜。
Plant Mol Biol. 1998 Sep;38(1-2):191-207.
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Localization of phosphatidylcholine in outer envelope membrane of spinach chloroplasts.菠菜叶绿体外被膜中磷脂酰胆碱的定位
J Cell Biol. 1985 May;100(5):1690-7. doi: 10.1083/jcb.100.5.1690.