Mackay C R, Marston W L, Dudler L, Hein W R
Basel Institute for Immunology, Switzerland.
Vet Immunol Immunopathol. 1991 Jan;27(1-3):183-8. doi: 10.1016/0165-2427(91)90098-w.
A 215 kDa molecule termed T19 marks CD4-CD8- T cells in sheep and cattle. In this report, we analysed the T19 or "null cell" panel of mAbs against gamma delta T cells of sheep, using a mAb specific for the gamma delta TCR. By two-colour immunofluorescence, all of the mAbs in the T19 panel reacted with gamma delta T cells or subsets thereof, although staining intensities and percentages of cells stained by the different mAbs indicated considerable heterogeneity for the T19 molecule. This probably results from differential expression of certain epitopes on T19. The reactivity of most of the mAbs for the 215 kDa T19 molecule was also confirmed by immunoprecipitation and SDS-PAGE.
一种名为T19的215 kDa分子标记绵羊和牛的CD4-CD8-T细胞。在本报告中，我们使用针对γδTCR的单克隆抗体，分析了针对绵羊γδT细胞的T19或“空细胞”单克隆抗体组。通过双色免疫荧光，T19组中的所有单克隆抗体均与γδT细胞或其子集发生反应，尽管不同单克隆抗体染色的细胞染色强度和百分比表明T19分子存在相当大的异质性。这可能是由于T19上某些表位的差异表达所致。免疫沉淀和SDS-PAGE也证实了大多数单克隆抗体对215 kDa T19分子的反应性。
