Mennel H D, Berweiler-Nippert U, Lorenz H, Geissel H
Department of Neuropathology, Medical Center of Pathology, University of Marburg, FRG.
Zentralbl Allg Pathol. 1990;136(7-8):671-8.
35 intracranial tumours, 18 gliomas, 12 meningiomas, one neurilemmoma (neurinoma), one malignant melanoma and two metastases were successfully grown in-vitro and were submitted to immunocytochemical reactions, including cytokeratin, glial fibrillary acid protein (GFAP), vimentin, fibronectin, S-100 protein, neurofilament proteins, neuron-specific enolase (NSE) and basic myelin protein (MBP). Cytokeratin in metastases, GFAP and vimentin in gliomas, vimentin in meningiomas were consistently positive. S-100 protein was weakly and partially positive in gliomas, meningiomas, the neurilemmoma and malignant melanoma. Positive demonstration of fibronectin within cells was interpreted as a consequence of phagocytosis, except in meningiomas where fibronectin expression next to cell membranes seemed genuine. All other tested markers proved negative. The most important result seems to be that cells expressed markers irrespective of cellular shape and cytological morphology. It can be concluded that the cellular population as a whole consisted of tumour cells during the short time under observation and that supportive cell contamination during this early growth period was negligible.
35例颅内肿瘤，包括18例胶质瘤、12例脑膜瘤、1例神经鞘瘤（神经瘤）、1例恶性黑色素瘤和2例转移瘤成功在体外培养，并进行了免疫细胞化学反应，检测指标包括细胞角蛋白、胶质纤维酸性蛋白（GFAP）、波形蛋白、纤连蛋白、S-100蛋白、神经丝蛋白、神经元特异性烯醇化酶（NSE）和碱性髓鞘蛋白（MBP）。转移瘤中的细胞角蛋白、胶质瘤中的GFAP和波形蛋白、脑膜瘤中的波形蛋白始终呈阳性。S-100蛋白在胶质瘤、脑膜瘤、神经鞘瘤和恶性黑色素瘤中呈弱阳性且部分阳性。细胞内纤连蛋白的阳性表现被解释为吞噬作用的结果，但在脑膜瘤中，细胞膜旁的纤连蛋白表达似乎是真实的。所有其他检测指标均为阴性。最重要的结果似乎是，细胞表达标记物与细胞形状和细胞学形态无关。可以得出结论，在观察的短时间内，整个细胞群体均由肿瘤细胞组成，并且在此早期生长阶段支持细胞的污染可忽略不计。
