Cheng X D, Lin S X, Shi J P, Wang Y L
Shanghai Institute of Biochemistry, Academia Sinica, PRC.
Sci China B. 1991 Mar;34(3):297-305.
The covalent modification of E. coli arginyl-tRNA synthetase by the 2',3'-dialdehyde derivative of tRNA(Arg) (tRNA(oxArg)) resulted in the complete inactivation of the ATP-PPi exchange and aminoacylation activities of the enzyme. Sodium dodecyl sulfate polyacrylamide gel electrophoresis of the ArgRS-tRNA(oxArg) covalent complexes indicated that two bands simultaneously appeared on the gel parallel with inactivation corresponding to different higher molecular weights. This result was different from that of the other aminoacyl-tRNA synthetase labeling systems as previously reported. Upon the ribonuclease treatment of the modified ArgRS, less than 15% of both the initial ATP-PPi exchange and aminocylation activities were recovered. During the whole process of labeling and RNase treatment, the two activities of the enzyme were closely associated.
tRNA(Arg)的2',3'-二醛衍生物（tRNA(oxArg)）对大肠杆菌精氨酰-tRNA合成酶的共价修饰导致该酶的ATP-PPi交换活性和氨酰化活性完全丧失。对精氨酰-tRNA合成酶（ArgRS）与tRNA(oxArg)的共价复合物进行十二烷基硫酸钠聚丙烯酰胺凝胶电泳，结果表明凝胶上同时出现两条与失活相对应的条带，其分子量较高且不同。这一结果与先前报道的其他氨酰-tRNA合成酶标记系统的结果不同。用核糖核酸酶处理修饰后的ArgRS后，初始ATP-PPi交换活性和氨酰化活性的恢复率均不到15%。在整个标记和核糖核酸酶处理过程中，该酶的这两种活性密切相关。
