Qin Guo-Hua, Meng Zi-Qiang
Institute of Environmental Medicine and Toxicology, Shanxi University, Taiyuan 030006, China.
Wei Sheng Yan Jiu. 2006 Sep;35(5):554-6.
To study the effects of sulfur dioxide (SO2) on activities and mRNA expression of hepatic and pulmonary cytochrome P4502B1 and 2E1 in rats.
Male Wistar rats were housed in exposure chambers and treated with 14, 28 and 56 mg/m3 SO2 for 6 h/d for 7 days, while control rats were exposed to filtered air in the same condition. The activities of CYP2E1 of rats were measured by spectrophotometry. Fluorescence spectrophotometry was used to study the activities of CYP2B1. The mRNAs of CYP2B1 and 2E1 were analyzed in livers and lungs by using a reverse transcription-polymerase chain reaction (RT-PCR) assay.
In the liver, decreases of CYP2B1 activity and mRNA were observed at higher dose of SO2 (28 and 56 mg/m3). However, the CYP2E1-dependent p-nitrophenol hydroxylases (p-NP) and mRNA were unaltered by SO2 at all detected concentrations. For lungs, CYP2B1 activity was unaltered by SO2 at low concentrations, except for a significant decrease in the rats exposed to SO2 at 56mg/m3, however, SO2 at higher concentrations (28 and 56 mg/m3) significantly decreased CYP2B1 mRNA. Significant inhibition of p-NP was observed in lungs of rats exposed to SO2 at 28 and 56 mg/m3. SO2 at higher concentrations (28 and 56 mg/m3) decreased significantly pulmonary CYP2E1 mRNA relative to control animals.
It was suggested that SO2 exposure could suppress the activities and mRNA expression of hepatic CYP2B1 and pulmonary CYP2B1 and 2E1 of rats.
研究二氧化硫（SO₂）对大鼠肝脏和肺组织中细胞色素P4502B1和2E1活性及mRNA表达的影响。
将雄性Wistar大鼠置于暴露箱中，分别用14、28和56mg/m³的SO₂处理，每天暴露6小时，持续7天，而对照大鼠在相同条件下暴露于过滤空气中。采用分光光度法测定大鼠CYP2E1的活性。用荧光分光光度法研究CYP2B1的活性。通过逆转录-聚合酶链反应（RT-PCR）分析肝脏和肺组织中CYP2B1和2E1的mRNA。
在肝脏中，较高剂量的SO₂（28和56mg/m³）可使CYP2B1活性和mRNA降低。然而，在所有检测浓度下，SO₂均未改变CYP2E1依赖的对硝基苯酚羟化酶（p-NP）和mRNA。对于肺组织，低浓度的SO₂对CYP2B1活性无影响，但在暴露于56mg/m³SO₂的大鼠中显著降低，然而，较高浓度（28和56mg/m³）的SO₂可显著降低CYP2B1 mRNA。在暴露于28和56mg/m³SO₂的大鼠肺组织中观察到对p-NP的显著抑制。相对于对照动物，较高浓度（28和56mg/m³）的SO₂可显著降低肺组织中CYP2E1 mRNA。
提示暴露于SO₂可抑制大鼠肝脏CYP2B1以及肺组织中CYP2B1和2E1的活性及mRNA表达。
