一些影响小鼠囊胚玻璃化冷冻成功的因素。

Some factors affecting successful vitrification of mouse blastocysts.

作者信息

Van Der Zwalmen P, Gaurois B, Ectors F J, Touati K, Massip A, Ectors F

机构信息

Department of Animal Reproduction, Faculty of Veterinary Medicine, Brussels, Belgium.

出版信息

Theriogenology. 1988 Dec;30(6):1177-83. doi: 10.1016/0093-691x(88)90293-2.

DOI:10.1016/0093-691x(88)90293-2

PMID:17087907

Abstract

The effects of temperature and exposure time to vitrification solutions on In vitro survival of mouse blastocysts were investigated. Blastocysts were first exposed for 10 min to vitrification Solution 1 (VS1) containing 10% glycerol-20% 1,2 propanediol in phosphate buffered saline (PBS), then to vitrification Solution 2 (VS2) with 25 % glycerol-25% 1,2 propanediol for various periods either at room temperature or at 4 degrees C. At room temperature survival dropped quickly, while at 4 degrees C an increase in survival was observed. It is concluded that the viability of mouse blastocyts after vitrification is dependent on the temperature and duration of equilibration in vitrification solutions.

摘要

研究了温度和暴露于玻璃化溶液的时间对小鼠囊胚体外存活的影响。囊胚首先在含有10%甘油-20% 1,2-丙二醇的磷酸盐缓冲盐水（PBS）中的玻璃化溶液1（VS1）中暴露10分钟，然后在室温或4℃下于含有25%甘油-25% 1,2-丙二醇的玻璃化溶液2（VS2）中暴露不同时间。在室温下存活率迅速下降，而在4℃下观察到存活率增加。得出结论，玻璃化后小鼠囊胚的活力取决于在玻璃化溶液中的平衡温度和持续时间。