人早期囊胚扩张期瘪胚的玻璃化冷冻：474 个周期的临床结局。
Vitrification of human early cavitating and deflated expanded blastocysts: clinical outcome of 474 cycles.
机构信息
Embryology Division, Krishna IVF Clinic, Zillaparishad Junction, Visakhapatnam, Pincode-530 002, Andhra Pradesh, India.
出版信息
J Assist Reprod Genet. 2009 Sep-Oct;26(9-10):523-9. doi: 10.1007/s10815-009-9356-0. Epub 2009 Oct 30.
PURPOSE
The present study was undertaken to evaluate and compare the post thaw survival, implantation and pregnancy rates of vitrified human early cavitating blastocysts with deflated expanded blastocysts.
MATERIAL AND METHODS
Supernumerary blastocysts were vitrified in 30% ethylene glycol-dimethyl sulphoxide based solution using cryoloop. Fully expanded blastocysts were deflated by gentle aspiration of the blastocoelic fluid using a micromanipulator until the cavity collapses prior to vitrification.
RESULTS
Of the 576 vitrified blastocysts, 545 (94.61%) survived thawing in the early cavitating blastocyst group which was significantly higher than deflated expanded blastocyst group, in which only 370 survived thawing out of 459 (80.62%). However, no significant difference was observed in implantation and pregnancy rates between early cavitating and deflated expanded blastocyst groups.
CONCLUSIONS
Early cavitating blastocyst would be the ideal stage for cryopreservation of human blastocysts as it has higher survival rate and avoids additional invasive procedures like deflation of the blastocoele.
目的
本研究旨在评估和比较玻璃化冷冻的早期囊胚期扩张囊胚与扩张囊胚期瘪陷囊胚的解冻后存活率、着床率和妊娠率。
材料和方法
使用 cryoloop 将多余的囊胚在 30%的乙二醇-二甲基亚砜基础溶液中进行玻璃化冷冻。在玻璃化冷冻之前，使用微操作器轻轻吸出囊胚腔中的液体，使完全扩张的囊胚瘪陷。
结果
在 576 个玻璃化冷冻的囊胚中，545 个（94.61%）在早期囊胚期瘪陷囊胚组中解冻后存活，这显著高于瘪陷扩张囊胚组，其中只有 370 个（80.62%）在 459 个瘪陷扩张囊胚中解冻后存活。然而，早期囊胚期瘪陷囊胚组与瘪陷扩张囊胚组的着床率和妊娠率没有显著差异。
结论
早期囊胚期瘪陷囊胚是人类囊胚冷冻的理想阶段，因为它具有更高的存活率，避免了额外的侵入性程序，如囊胚腔瘪陷。
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