Kubler-Kielb Joanna, Vinogradov Evgeny, Chu Chiayung, Schneerson Rachel
National Institute of Child Health and Human Development, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892, USA.
Carbohydr Res. 2007 Feb 26;342(3-4):643-7. doi: 10.1016/j.carres.2006.09.017. Epub 2006 Nov 7.
Shigella flexneri causes diarrheal diseases especially in infants and children in developing countries. Modifications of the lipopolysaccharide (LPS) molecule, like bacteriophage-mediated glucosylation and acetylation of the O-specific chain (O-SP), are important for the LPS antigenicity and consequently for the immunogenicity of the polysaccharide-based vaccines against shigellosis. Here, we report the degree of O-acetylation and the localisation of O-acetyl groups and side-chain glucose substitution in the O-SP (scheme) in different preparations of S. flexneri type 2a LPS. [structure: see text]
福氏志贺菌尤其会在发展中国家的婴幼儿中引发腹泻疾病。脂多糖(LPS)分子的修饰,如噬菌体介导的O-特异性链(O-SP)的糖基化和乙酰化,对于LPS的抗原性以及基于多糖的志贺菌病疫苗的免疫原性都很重要。在此,我们报告了福氏2a型LPS不同制剂中O-乙酰化程度以及O-乙酰基团和O-SP中侧链葡萄糖取代的定位(示意图)。[结构:见正文]