Wright Karen, Guerreiro Catherine, Laurent Isabelle, Baleux Françoise, Mulard Laurence A
Unité de Chimie Organique, URA CNRS 2128, Institut Pasteur, 28 rue du Dr Roux, 75 724, Paris15, France.
Org Biomol Chem. 2004 May 21;2(10):1518-27. doi: 10.1039/b400986J. Epub 2004 Apr 26.
The synthesis of three neoglycopeptides incorporating carbohydrate haptens, differing in length, covalently linked to a non natural universal T helper peptide is disclosed. They were synthesized according to a blockwise strategy based on the condensation of appropriate di-, tri-, and tetrasaccharide trichloroacetimidate donors onto an azidoethyl 2-acetamido-2-deoxybeta-D-glucopyranoside acceptor. Use of thiol-maleimide coupling chemistry allowed site-selective efficient conjugation.
公开了三种结合了碳水化合物半抗原的新糖肽的合成方法，这些半抗原长度不同，通过共价键与一种非天然通用T辅助肽相连。它们是根据逐步策略合成的，该策略基于将合适的二糖、三糖和四糖三氯乙酰亚胺供体缩合到叠氮乙基2-乙酰氨基-2-脱氧-β-D-吡喃葡萄糖苷受体上。使用硫醇-马来酰亚胺偶联化学实现了位点选择性高效共轭。
