Matrosovich M N, Gambaryan A S, Reizin F N, Chumakov M P
Institute of Poliomyelitis and Viral Encephalitides, USSR Academy of Medical Sciences, Moscow Region.
Virology. 1991 Jun;182(2):879-82. doi: 10.1016/0042-6822(91)90634-n.
To evaluate the recognition by influenza viruses of the C9-C7 polyhydroxylated moiety of sialic acid (SA) receptor determinant a novel assay has been developed based on the assessment of binding by the solid-phase immobilized virus of the enzyme-labeled sialyglycoprotein fetuin treated by periodate or periodate/borohydride to contain an 8-carbon aldehyde, 7-carbon aldehyde, or corresponding hydroxyl analogues of SA. Some features of recognition by human influenza viruses of these SA analogues were type and subtype specific, especially marked differences being found between type A and type B viruses. At the same time a significant diversity was observed among virus strains belonging to the same subtype. The assay described provides a new tool for the differentiation of influenza viruses according to receptor binding properties and for an investigation of molecular interactions in the receptor binding site of the virus.
为了评估流感病毒对唾液酸（SA）受体决定簇的C9 - C7多羟基化部分的识别情况，基于对经高碘酸盐或高碘酸盐/硼氢化物处理以含有8碳醛、7碳醛或相应SA羟基类似物的酶标记唾液糖蛋白胎球蛋白与固相固定病毒结合的评估，开发了一种新的检测方法。人类流感病毒对这些SA类似物的识别的一些特征具有型别和亚型特异性，在A型和B型病毒之间发现了特别显著的差异。同时，在属于同一亚型的病毒株之间观察到了显著的多样性。所描述的检测方法为根据受体结合特性区分流感病毒以及研究病毒受体结合位点中的分子相互作用提供了一种新工具。
