Yang Xuan, Meier John, Chang Lina, Rowan Michael, Baumann Paul C
School of Natural Resources, The Ohio State University, 2021 Coffey Road, Columbus, Ohio 43210, USA.
Environ Toxicol Chem. 2006 Nov;25(11):3035-8. doi: 10.1897/05-706r.1.
The Comet assay was used to compare levels of DNA damage in brown bullheads (Ameiurus nebulosus) collected from three known contaminated locations, the Cuyahoga River (OH, U.S.A.), Ashtabula River (OH, U.S.A.; both tributaries to Lake Erie, USA), and Ashumet Pond (Cape Cod, MA, U.S.A.), with brown bullheads collected from three paired reference sites, Old Woman Creek (OH, U.S.A.), Conneaut River (OH, U.S.A.; both tributaries to Lake Erie), and Great Herring Pond (mainland MA, U.S.A.), respectively. Blood was sampled from each fish, and the Comet assay was conducted on erythrocytes. The assay results demonstrate that fish from the three contaminated sites each suffered higher DNA damage compared with fish from their respective reference sites. The results also show that the genetic damage was associated with the occurrence of external lesions and deformities in fish. The Comet assay is sufficiently sensitive to detect exposure of natural fish populations to environmental levels of genotoxic contaminants.
彗星试验被用于比较从三个已知受污染地点采集的褐首鲶（Ameiurus nebulosus）的DNA损伤水平，这三个地点分别是凯霍加河（美国俄亥俄州）、阿什塔比拉河（美国俄亥俄州，均为美国伊利湖的支流）和阿舒梅特池塘（美国马萨诸塞州科德角），同时与从三个配对的参考地点采集的褐首鲶进行比较，这三个参考地点分别是老妇溪（美国俄亥俄州）、康纳奥特河（美国俄亥俄州，均为伊利湖的支流）和大鲱鱼池塘（美国马萨诸塞州大陆）。从每条鱼身上采集血液，并对红细胞进行彗星试验。试验结果表明，来自三个受污染地点的鱼与各自参考地点的鱼相比，均遭受了更高程度的DNA损伤。结果还表明，遗传损伤与鱼的外部损伤和畸形的发生有关。彗星试验足够灵敏，能够检测自然鱼类种群接触环境水平的遗传毒性污染物的情况。
