Kudo Noboru, Kuratomi Kanta, Hatada Nobuko, Ikadai Hiromi, Oyamada Takashi
Department of Veterinary Parasitology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori 034-8628, Japan.
J Parasitol. 2005 Aug;91(4):750-5. doi: 10.1645/GE-3441.1.
Third-stage larvae of Gongylonema pulchrum from naturally infected dung beetles were inoculated orally into 24 rabbits. Worm recovery ranged from 54 to 91% (mean = 67.5%) during the period from 24 hr to 52 wk postinoculation (PI). Two hours PI, the larvae entered the mucosa at the junction of the stomach and esophagus and migrated upward. Early development occurred primarily in pharyngeal mucosa, tongue, and buccal mucosa. The third molt took place 11 days PI and the final molt at 36 days PI. Male worms reached sexual maturity at 7 wk PI and females at 9 wk PI. Adult worms were found mainly in the esophagus but also occurred in the tongue and the wall of the oral cavity after 30 wk PI. Embryonated eggs appeared in the feces of 3 rabbits inoculated with 50 or 100 larvae on days 72-81 PI. Morphologically, the cuticle in young fourth-stage larvae exhibited bosses on the anterior portion on day 11 PI, and the left spicule length : total body length exhibited no remarkable change between 9 and 52 wk PI. The latter finding confirms the utility of the ratio for identification of the nematode.
将自然感染的蜣螂体内的美丽筒线虫第三期幼虫经口接种到24只兔子体内。在接种后24小时至52周期间，蠕虫回收率为54%至91%（平均 = 67.5%）。接种后2小时，幼虫进入胃和食管交界处的黏膜并向上迁移。早期发育主要发生在咽黏膜、舌和颊黏膜。第三次蜕皮发生在接种后11天，最后一次蜕皮发生在接种后36天。雄虫在接种后7周达到性成熟，雌虫在接种后9周达到性成熟。成年虫主要见于食管，但在接种后30周后也见于舌和口腔壁。在接种50或100条幼虫的3只兔子的粪便中，在接种后72 - 81天出现了含胚卵。形态学上，接种后11天，年轻的第四期幼虫的角质层在前部有凸起，接种后9周和52周之间，左交合刺长度与虫体总长度之比无明显变化。后一发现证实了该比例在鉴定线虫方面的实用性。
