León-Régagnon Virginia, Guillén-Hernández Sergio, Arizmendi-Espinosa María Antonieta
Departamento de Zoología, Instituto de Biología, UNAM. Apdo, Postal 70-153, C.P. 04510, México, DF México.
J Parasitol. 2005 Aug;91(4):915-21. doi: 10.1645/GE-430R.1.
Haematoloechus floedae was originally described from the lungs of Rana catesbeiana in Texas, and later reported in Florida, in the United States. It was proposed to be synonymous with H. breviplexus, H. parviplexus, and H. varioplexus. We obtained specimens of H. floedae from Rana brownorum and R. vaillanti in Yucatán, Mexico; R. cf. forreri and R. taylori in Guanacaste, Costa Rica; and R. catesbeiana in Georgia and California. Some specimens were processed for morphological study; sequences of the 28S of the rDNA and the mitochondrial COI were obtained from several specimens of each population. Phylogenetic analysis of molecular data indicates studied populations constitute a single taxon, different from H. varioplexus, H. breviplexus, and H. parviplexus. Some morphological characters remain constant among populations of H. floedae, and are useful for differentiating this species. These include the oral sucker-pharynx ratio, oral sucker-ventral sucker ratio, ovary and testes shape, extension of uterine longitudinal loops, and extension of vitelline follicles (in fully developed worms). Sequence homogeneity among populations of H. floedae suggests a recent spread, perhaps due to the introduction of R. catesbeiana for culture to the western United States, southeast Mexico, and Costa Rica.
弗氏血蛭最初是从美国得克萨斯州的牛蛙肺中描述的，后来在美国佛罗里达州也有报道。有人提议将其与短支血蛭、小支血蛭和多支血蛭同义。我们从墨西哥尤卡坦半岛的棕蛙和瓦氏蛙、哥斯达黎加瓜纳卡斯特省的疑似福氏蛙和泰勒蛙以及佐治亚州和加利福尼亚州的牛蛙中获得了弗氏血蛭的标本。一些标本经过处理用于形态学研究；从每个种群的几个标本中获得了核糖体DNA的28S和线粒体细胞色素氧化酶亚基I（COI）的序列。分子数据的系统发育分析表明，所研究的种群构成一个单一的分类单元，与多支血蛭、短支血蛭和小支血蛭不同。弗氏血蛭种群中的一些形态特征保持不变，有助于区分该物种。这些特征包括口吸盘与咽的比例、口吸盘与腹吸盘的比例、卵巢和睾丸的形状、子宫纵袢的延伸以及卵黄滤泡的延伸（在完全成熟的虫体中）。弗氏血蛭种群之间的序列同质性表明其近期有扩散现象，可能是由于牛蛙被引入到美国西部、墨西哥东南部和哥斯达黎加进行养殖所致。
