Tuttle Allison D, Law J Mac, Harms Craig A, Lewbart Gregory A, Harvey Stephen B
Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina, USA.
J Am Assoc Lab Anim Sci. 2006 Nov;45(6):22-6.
Surgical harvest of Xenopus laevis oocytes for developmental research is a common procedure that requires closure of a 0.5- to 2.0-cm incision with suture material. Although such harvests are a frequent practice, little published information exists to provide guidance regarding the most appropriate suture material for wound closure in laboratory amphibians. To determine which suture material elicits the least response in amphibian skin, we used Xenopus laevis as a model to investigate the gross and histologic tissue reactions to 5 commonly used suture materials-3-0 silk, monofilament nylon, polydioxanone, polyglactin 910, and chromic gut. The skin reacted in 3 ways to suture material, showing edema, epidermal changes, and inflammation. Although the gross reactions to monofilament nylon, polydioxanone, and polyglactin 910 were clinically indistinguishable and were associated with lowest gross reaction scores, monofilament nylon elicited the least histologic reaction and therefore seems to be the most appropriate choice for use in amphibian skin.
为进行发育研究而从非洲爪蟾获取卵母细胞的手术是一种常见操作，需要用缝合材料闭合0.5至2.0厘米的切口。尽管这种取材操作很常见，但关于实验室两栖动物伤口闭合最合适的缝合材料，几乎没有公开信息可供指导。为了确定哪种缝合材料在两栖动物皮肤中引起的反应最小，我们以非洲爪蟾为模型，研究了对5种常用缝合材料（3-0丝线、单丝尼龙、聚二氧六环酮、聚乙醇酸910和铬制肠线）的大体和组织学组织反应。皮肤对缝合材料有3种反应方式，表现为水肿、表皮变化和炎症。尽管单丝尼龙、聚二氧六环酮和聚乙醇酸910的大体反应在临床上难以区分，且与最低的大体反应评分相关，但单丝尼龙引起的组织学反应最小，因此似乎是用于两栖动物皮肤的最合适选择。
