Lee Jung-Bok, Sohn Ho-Yong, Shin Kee-Sun, Jo Min-Sub, Kim Jang-Eok, Lee Se-Won, Shin Ji-Won, Kum Eun-Joo, Kwon Gi-Seok
School of Bioresource Sciences, Andong National University, Andong 760-749, South Korea.
J Agric Food Chem. 2006 Nov 15;54(23):8824-8. doi: 10.1021/jf061276e.
Endosulfan, an endocrine disrupting chemical, is a widely used cyclodiene organochlorine pesticide worldwide, and it blocks neuronal GABA(A)-gated chloride channels in mammals and aquatic organisms. Endosulfan and its metabolites, such as endosulfan sulfate, are persistent in environments and are considered as toxic chemicals. For bioremediation of endosulfan, in this study, an attempt was made to isolate an endosulfan and endosulfan sulfate degrading bacterium from endosulfan-polluted agricultural soil. Through repetitive enrichment and successive subculture using endosulfan or endosulfan sulfate as the sole carbon source, a bacterium KS-2P was isolated. The KS-2P was identified as Pseudomonas sp. on the basis of the results of a 16S rDNA sequencing analysis and MIDI test. The degradation ratios for endosulfan or endosulfan sulfate in minimal medium containing endosulfan (23.5 microg mL(-1)) or endosulfan sulfate (21 microg mL(-1)) were 52% and 71%, respectively. Our results suggest that Pseudomonas sp. KS-2P has potential as a biocatalyst for endosulfan bioremediation.
硫丹是一种内分泌干扰化学物质，是一种在全球广泛使用的环二烯有机氯农药，它会阻断哺乳动物和水生生物体内神经元的GABA(A)门控氯离子通道。硫丹及其代谢产物，如硫丹硫酸盐，在环境中具有持久性，被视为有毒化学品。为了对硫丹进行生物修复，在本研究中，尝试从受硫丹污染的农业土壤中分离出一种能降解硫丹和硫丹硫酸盐的细菌。通过以硫丹或硫丹硫酸盐作为唯一碳源进行重复富集和连续传代培养，分离出了一株细菌KS-2P。根据16S rDNA测序分析和MIDI测试结果，KS-2P被鉴定为假单胞菌属。在含有硫丹（23.5微克/毫升）或硫丹硫酸盐（21微克/毫升）的基本培养基中，硫丹或硫丹硫酸盐的降解率分别为52%和71%。我们的结果表明，假单胞菌属KS-2P有潜力作为硫丹生物修复的生物催化剂。
