Environmental Biotechnology Division, Indian Institute of Toxicology Research (Council of Scientific & Industrial Research), Lucknow, Uttar Pradesh, India.
J Appl Microbiol. 2010 Dec;109(6):2135-43. doi: 10.1111/j.1365-2672.2010.04845.x. Epub 2010 Sep 6.
To isolate bacteria capable of degrading endosulfan (ES) and the more toxic ES sulfate and to characterize their metabolites.
A Pseudomonas sp. strain IITR01 capable of degrading α-ES and toxic ES sulfate was isolated using technical-ES through enrichment culture techniques. No growth and no degradation were observed using β-ES. Thin-layer chromatography and gas chromatography-mass spectrum analysis revealed the disappearance of both α-ES and ES sulfate and the formation of hydroxylated products ES diol, ether and lactone. We show here for the first time the formation of aforementioned metabolites in contrast to ES hemisulfate yielded by an Arthrobacter sp. Metabolism of α-ES and endosulfate was also observed using the crude cell extract of IITR01. The molecular mass of protein induced during the degradation of α-ES and sulfate as substrate was found to be approximately 150 kDa as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
We describe characterization of bacterium capable of degrading α-ES and ES sulfate but not β-ES. Genetic investigation suggests that a gene nonhomologous to the reported esd may be present in the strain IITR01.
This study describes toxic ES degradation by a Pseudomonas species that may be utilized for the bioremediation of the industrial soils contaminated with ES residues.
分离能够降解硫丹（ES）及其毒性更大的硫酸酯的细菌，并对其代谢产物进行表征。
通过富集培养技术，从技术 ES 中分离出一种能够降解α-ES 和毒性 ES 硫酸酯的假单胞菌 IITR01 菌株。β-ES 则没有观察到生长和降解。薄层层析和气相色谱-质谱分析显示，α-ES 和 ES 硫酸酯都消失了，并形成了羟基化产物 ES 二醇、醚和内酯。与节杆菌产生的 ES 半硫酸盐相比，我们首次在这展示了上述代谢产物的形成。用 IITR01 的粗细胞提取物也观察到了α-ES 和内硫酸盐的代谢。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）测定，发现降解α-ES 和硫酸盐作为底物时诱导的蛋白质分子量约为 150kDa。
我们描述了一种能够降解α-ES 和 ES 硫酸酯但不能降解β-ES 的细菌的特征。遗传研究表明，IITR01 菌株中可能存在与报道的 esd 非同源的基因。
本研究描述了一种假单胞菌对毒性 ES 的降解，可用于生物修复受 ES 残留污染的工业土壤。
