氧化葡萄糖酸杆菌IFO 3244中依赖NADP的莽草酸脱氢酶的纯化、性质及其在酶法生产莽草酸中的应用
Purification and properties of NADP-dependent shikimate dehydrogenase from Gluconobacter oxydans IFO 3244 and its application to enzymatic shikimate production.
作者信息
Adachi Osao, Ano Yoshitaka, Toyama Hirohide, Matsushita Kazunobu
机构信息
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan.
出版信息
Biosci Biotechnol Biochem. 2006 Nov;70(11):2786-9. doi: 10.1271/bbb.60305. Epub 2006 Nov 7.
NADP-Dependent shikimate dehydrogenae (SKDH, EC 1.1.1.25) was purified from Gluconobacter oxydans IFO 3244. SKDH showed a single protein band on native-PAGE accompanying enzyme activity. It required NADP exclusively and catalyzed only the shuttle reaction between shikimate and 3-dehydroshikimate. The optimum pH for shikimate oxidation and 3-dehydroshikimate reduction was found at pH 10 and 7 respectively. SKDH proved to be a useful catalyst for shikimate production from 3-dehydroshikimate.
从氧化葡萄糖酸杆菌IFO 3244中纯化出依赖于烟酰胺腺嘌呤二核苷酸磷酸（NADP）的莽草酸脱氢酶（SKDH，EC 1.1.1.25）。SKDH在非变性聚丙烯酰胺凝胶电泳（native-PAGE）上呈现出一条伴随酶活性的单一蛋白条带。它仅需要NADP，并仅催化莽草酸和3-脱氢莽草酸之间的穿梭反应。发现莽草酸氧化和3-脱氢莽草酸还原的最适pH分别为10和7。SKDH被证明是从3-脱氢莽草酸生产莽草酸的有用催化剂。
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