Fei Qin-Ming, Jiang Xiao-Xing, Chen Tong-Yi, Li Jun, Murakami Hideki, Tsai Kai-Jow, Hutton William C
Department of Orthopaedics, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Acta Biochim Biophys Sin (Shanghai). 2006 Nov;38(11):773-9. doi: 10.1111/j.1745-7270.2006.00230.x.
In order to compare the difference between young and old intervertebral disc cells and their responsiveness to recombinant human bone morphogenetic protein-2 (rhBMP-2), disc cells were isolated from the anulus fibrosus (AF) and transition zones of lumbar discs from eight old and eight young New Zealand white rabbits. Compared with the cells from the young rabbits, cells from old rabbits respond less to rhBMP-2 treatment with respect to sulfated-glycosaminoglycan (sGAG) synthesis and aggrecan gene expression. But in collagen I and collagen II gene expressions, there are no significant differences between the old and the young. When comparing sGAG content, aggrecan, and collagen II gene expression of the old AF cells after rhBMP-2 treatment with that of the young AF cells without rhBMP-2 treatment, the old AF cells with rhBMP-2 treatment have a greater capacity to synthesize sGAG bound in the cells and to release sGAG in the media, as well as to express aggrecan and collagen II gene. It can be concluded that old AF cells after rhBMP-2 treatment have a greater capacity to synthesize sGAG and express aggrecan and collagen II as compared to young AF cells without rhBMP-2 treatment. Thus rhBMP-2 can reverse the decline in the anabolic capacity of the disc cells with ageing. So it seems that rhBMP-2 has potential for use as an agent to retard a key component of disc degeneration and loss of disc matrix.
为了比较年轻和年老椎间盘细胞之间的差异及其对重组人骨形态发生蛋白-2（rhBMP-2）的反应性，从8只老年和8只年轻新西兰白兔的腰椎间盘纤维环（AF）和过渡区分离出椎间盘细胞。与年轻兔子的细胞相比，老年兔子的细胞在硫酸化糖胺聚糖（sGAG）合成和聚集蛋白聚糖基因表达方面对rhBMP-2治疗的反应较小。但在I型胶原蛋白和II型胶原蛋白基因表达方面，老年和年轻细胞之间没有显著差异。当比较rhBMP-2处理后的老年AF细胞与未用rhBMP-2处理的年轻AF细胞的sGAG含量、聚集蛋白聚糖和II型胶原蛋白基因表达时，经rhBMP-2处理的老年AF细胞在合成细胞内结合的sGAG、向培养基中释放sGAG以及表达聚集蛋白聚糖和II型胶原蛋白基因方面具有更大的能力。可以得出结论，与未用rhBMP-2处理的年轻AF细胞相比，rhBMP-2处理后的老年AF细胞在合成sGAG、表达聚集蛋白聚糖和II型胶原蛋白方面具有更大的能力。因此，rhBMP-2可以逆转椎间盘细胞随着衰老而出现的合成代谢能力下降。所以rhBMP-2似乎有潜力作为一种药物来延缓椎间盘退变和椎间盘基质丢失这一关键过程。
