一种确定大分子自旋标记特异性的动力学方法。
A kinetic method to establish the specificity of spin labelling of macromolecules.
作者信息
Griffiths J R, Price N C, Radda G K
出版信息
Biochem J. 1975 Jun;147(3):609-12. doi: 10.1042/bj1470609.
Abstract
A method has been devised for establishing the specificity of spin labelling of a macromolecule. Analysis of the rate of incorporation of spin label under different conditions permits the assignment of reacting groups to classes of differing reactivity. The method is illustrated for the enzyme glycogen phosphorylase b.
摘要
已经设计出一种用于确定大分子自旋标记特异性的方法。分析在不同条件下自旋标记的掺入速率，可以将反应基团归为具有不同反应活性的类别。以糖原磷酸化酶b为例对该方法进行了说明。
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本文引用的文献
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
The reactivity of thiol groups in aldolase.醛缩酶中硫醇基团的反应活性。
Biochem Soc Symp. 1970;31:49-58.
3
The reactivity of group-specific chromophoric reagents.组特异性发色试剂的反应性。
Biochem Soc Symp. 1970;31:147-55.
4
The sulfhydryl groups of muscle phosphorylase. V. The reactive sulfhydryl peptides.肌肉磷酸化酶的巯基。V. 活性巯基肽。
Can J Biochem. 1970 Jul;48(7):763-76. doi: 10.1139/o70-120.
5
Improvement of the spin-labelling technique by the use of a radioactive spin label.通过使用放射性自旋标记改进自旋标记技术。
FEBS Lett. 1973 Nov 1;36(3):351-4. doi: 10.1016/0014-5793(73)80408-9.
6
Studies on the interaction of ligands with phosphorylase b using a spin-label probe.使用自旋标记探针研究配体与磷酸化酶b的相互作用。
Eur J Biochem. 1972 Oct;30(2):339-47. doi: 10.1111/j.1432-1033.1972.tb02103.x.
7
Probes for the conformational transitions of phosphorylase b. Effect of ligands studied by proton relaxation enhancement, fluorescence and chemical reactivities.磷酸化酶b构象转变的探针。通过质子弛豫增强、荧光和化学反应性研究配体的作用。
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Magnetic resonance studies of spin-labeled creatine kinase system and interaction of two paramagnetic probes.自旋标记肌酸激酶系统的磁共振研究及两种顺磁探针的相互作用。
Proc Natl Acad Sci U S A. 1969 Sep;64(1):219-26. doi: 10.1073/pnas.64.1.219.
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