Griffiths J R, Price N C, Radda G K
Biochem J. 1975 Jun;147(3):609-12. doi: 10.1042/bj1470609.
A method has been devised for establishing the specificity of spin labelling of a macromolecule. Analysis of the rate of incorporation of spin label under different conditions permits the assignment of reacting groups to classes of differing reactivity. The method is illustrated for the enzyme glycogen phosphorylase b.
已经设计出一种用于确定大分子自旋标记特异性的方法。分析在不同条件下自旋标记的掺入速率,可以将反应基团归为具有不同反应活性的类别。以糖原磷酸化酶b为例对该方法进行了说明。