小鼠干扰素诱导的2',5'-寡腺苷酸依赖性核糖核酸酶L（RNase L）基因的组织
Organization of the interferon-inducible 2',5'-oligoadenylate-dependent ribonuclease L (RNase L) gene of mouse.
作者信息
Pandey Mitali, Rath Pramod C
机构信息
Molecular Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
出版信息
Mol Biol Rep. 2007 Jun;34(2):97-104. doi: 10.1007/s11033-006-9020-z. Epub 2006 Nov 8.
Interferons (IFNs) induce a 2',5'-oligoadenylate (2-5A)-dependent ribonuclease L (RNase L) following virus-infection of mammalian cells. RNase L degrades both viral and cellular RNAs and restricts virus-proliferation. We have studied organization of RNase L gene in genomic DNA from the mouse liver by Southern blot analysis. Several BamHI, BglII, EcoRI, HincII, HindIII, NcoI, PstI, SacI, and XbaI restriction fragments hybridized to (32)P-labeled mouse RNase L cDNA and the 5'-proximal exon probes. Mouse RNase L gene exists as a single copy (>16 kb DNA) gene. A 5 kb HindIII and a 2.5 kb EcoRI DNA were detected as 5'-upstream DNA of the gene which may contain mouse RNase L promoter. Our results will help studying mouse RNase L gene promoter in further details.
干扰素（IFNs）在哺乳动物细胞受到病毒感染后会诱导产生一种依赖2',5'-寡腺苷酸（2-5A）的核糖核酸酶L（RNase L）。RNase L会降解病毒RNA和细胞RNA，从而限制病毒增殖。我们通过Southern印迹分析研究了小鼠肝脏基因组DNA中RNase L基因的组织情况。几个BamHI、BglII、EcoRI、HincII、HindIII、NcoI、PstI、SacI和XbaI限制性片段与（32）P标记的小鼠RNase L cDNA及5'-近端外显子探针杂交。小鼠RNase L基因以单拷贝（>16 kb DNA）基因形式存在。一个5 kb的HindIII片段和一个2.5 kb的EcoRI片段被检测为该基因的5'-上游DNA，其可能包含小鼠RNase L启动子。我们的结果将有助于进一步详细研究小鼠RNase L基因启动子。
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