Hu Kejin
Pharmacology Department, University of Pittsburgh, W1301, BST, 200 Lothrop Street, PA 15213, USA.
FEBS Lett. 2006 Nov 27;580(27):6361-5. doi: 10.1016/j.febslet.2006.10.048. Epub 2006 Nov 7.
Mechanisms for loss and gain of introns are elusive. Reported here is a new pattern of intron loss which features a random loss of a single intron in a multiple-intron gene with its neighboring introns remained, which process is defined as intron exclusion. Intron exclusion is reminiscent of removal of a limited stretch of non-homologous sequence in a homologous recombination (HR) triggered by a double strand break (DSB), and therefore lends further evidence for a theory of intron loss through HR between a cDNA and its genomic intron-containing locus. Thus, a model for intron loss is formulated.
内含子丢失和获得的机制尚不清楚。本文报道了一种新的内含子丢失模式，其特征是多内含子基因中单个内含子随机丢失，而其相邻内含子保留，这一过程被定义为内含子排除。内含子排除让人联想到由双链断裂（DSB）引发的同源重组（HR）中有限长度非同源序列的去除，因此进一步为通过cDNA与其含基因组内含子位点之间的HR导致内含子丢失的理论提供了证据。由此，构建了一个内含子丢失模型。
