Bozzi Fabio, Gambirasio Felicita, Luksch Roberto, Collini Paola, Brando Bruno, Fossati-Bellani Franca
Department of Medical Oncology, Division of Pediatrics, "C" Istituto Nazionale per lo Studio e la Cura dei Tumori, Milan, Italy.
Anticancer Res. 2006 Sep-Oct;26(5A):3281-7.
In order to identify neuroblastoma cells infiltrating the bone marrow, a triple-color flow-cytometric assay was developed combining CD56 and CD45 with the intracellular anti-NB84 specific antibody.
The bilateral aspirates obtained from 27 consecutive children over the age of one year with stage 4 neuroblastoma were evaluated.
Neuroblastoma cells were detected in the bone marrow of 17/27 (63%) and 19/27 (70%) cases using cytomorphology and triple-color flow-cytometry, respectively. Using cytometry, the percentage of CD56+/NB84+/CD45-cells infiltrating the bone marrow ranged from 0.02% to 65%. Five out of eight patients without bone marrow involvement according to cytometry are in continuous complete remission, while only 3 out of 19 patients whose bone marrow gave positive results are still alive.
By combining CD45 and CD56 with the specific antibody, NB84, directed against neuroblastoma cells, we developed a rapid and reliable cytometric assay that can be associated with conventional cytomorphological bone marrow evaluation to detect infiltrating neuroblastoma cells, especially in cases of dubious positivity.
为了识别浸润骨髓的神经母细胞瘤细胞，开发了一种三色流式细胞术检测方法，将CD56和CD45与细胞内抗NB84特异性抗体相结合。
对27例年龄超过1岁的4期神经母细胞瘤患儿的双侧骨髓穿刺液进行评估。
分别采用细胞形态学和三色流式细胞术在17/27（63%）和19/27（70%）例患儿的骨髓中检测到神经母细胞瘤细胞。采用流式细胞术，浸润骨髓的CD56+/NB84+/CD45-细胞百分比为0.02%至65%。根据流式细胞术，8例无骨髓受累的患者中有5例持续完全缓解，而19例骨髓检查结果为阳性的患者中只有3例仍存活。
通过将CD45和CD56与针对神经母细胞瘤细胞的特异性抗体NB84相结合，我们开发出一种快速可靠的流式细胞术检测方法，该方法可与传统的细胞形态学骨髓评估相结合，以检测浸润性神经母细胞瘤细胞，尤其是在阳性结果存疑的病例中。
