Fukuoka Tomoki, Hibi Kenji, Nakao Akimasa
Gastroenterological Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8560, Japan.
Anticancer Res. 2006 Sep-Oct;26(5A):3333-5.
The combined methylation status of the p16, helicase-like transcription factor (HLTF) and T-cadherin, H-cadherin (CDH13) genes in 35 resected primary esophageal squamous cell carcinoma (ESCC) was examined using methylation-specific PCR. To examine whether the methylation status could be a marker for the malignancy of ESCC, the methylation status was correlated with the clinicopathological features of the affected patients. Aberrant methylation of the p16, HLTF and CDH13 genes was detected in 28 (80%), one (3%) and five (14%) out of 35 ESCC specimens, respectively. ESCC with methylation-positive genes showed a trend towards larger tumor size and deeper invasion (p = 0.139 and p = 0.0664, respectively). Thus, the methylation of the p16, HLTF and CDH13 genes is a high malignancy factor in ESCC.
采用甲基化特异性聚合酶链反应（PCR）检测了35例手术切除的原发性食管鳞状细胞癌（ESCC）中p16、解旋酶样转录因子（HLTF）和T-钙黏蛋白、H-钙黏蛋白（CDH13）基因的联合甲基化状态。为了检验甲基化状态是否可作为ESCC恶性程度的标志物，将甲基化状态与受累患者的临床病理特征进行了关联分析。在35例ESCC标本中，分别有28例（80%）、1例（3%）和5例（14%）检测到p16、HLTF和CDH13基因的异常甲基化。甲基化阳性基因的ESCC显示出肿瘤体积更大和浸润更深的趋势（分别为p = 0.139和p = 0.0664）。因此，p16、HLTF和CDH13基因的甲基化是ESCC的一个高恶性因素。
