Zuzak T J, Rist L, Eggenschwiler J, Grotzer M A, Viviani A
University of Applied Sciences, Waedenswil, Switzerland.
Anticancer Res. 2006 Sep-Oct;26(5A):3485-92.
Medulloblastoma constitute more than 20% of all paediatric brain tumours and are the most common malignant brain tumours in children. Adjuvant chemotherapy has seen a strong increase in the use of complementary medicine for cancer treatment. Evidence for cytotoxic and apoptotic effects of Viscum album (Mistletoe) in vitro is available, however, no data concerning paediatric tumours, especially paediatric brain tumours, has been provided so far.
In order to compare the receptiveness of medulloblastoma cells to different Viscum album preparations, in vitro cytotoxic effects of eight Viscum album extracts on four different paediatric medulloblastoma cell lines were analysed by MTT-Tests. Lectin contents of the extracts were determined to correlate them with the mitochondrial activity of mistletoe-treated cells. Flowcytometric analyses with Annexin V-FITC staining were carried out to quantify the amount of apoptotic cells compared to necrotic and viable cells.
Data obtained with the medulloblastoma cell lines, Daoy, D342, D425 and UW-288-2, treated with Viscum album preparations from eight dissimilar host trees (Iscucin Abietis, Pini, Populi, Mali, Salicis, Crataegi, Quercus and Tiliae), indicated a significant growth-inhibition of all cell lines, yet the cell susceptibility was dissimilar against the different extracts. The decrease in mitochondrial activity and increase in apoptosis correlated with the lectin content of the used preparation in a dose-dependent manner.
These in vitro results show that paediatric medulloblastoma cells respond to Viscum album preparations, by undergoing cell death through apoptosis and that this growth-inhibition correlates with the lectin content of the used preparation.
髓母细胞瘤占所有儿童脑肿瘤的20%以上，是儿童最常见的恶性脑肿瘤。辅助化疗中，用于癌症治疗的补充药物的使用量大幅增加。体外实验已证实欧洲槲寄生具有细胞毒性和凋亡作用，然而，目前尚无关于儿科肿瘤，尤其是儿童脑肿瘤的数据。
为比较髓母细胞瘤细胞对不同欧洲槲寄生制剂的反应性，通过MTT试验分析了八种欧洲槲寄生提取物对四种不同儿童髓母细胞瘤细胞系的体外细胞毒性作用。测定提取物中的凝集素含量，以将其与经槲寄生处理的细胞的线粒体活性相关联。采用膜联蛋白V-FITC染色进行流式细胞术分析，以量化凋亡细胞与坏死细胞和活细胞相比的数量。
用来自八种不同寄主树（欧洲冷杉、松树、杨树、苹果树、柳树、山楂树、橡树和菩提树）的欧洲槲寄生制剂处理髓母细胞瘤细胞系Daoy、D342、D425和UW-288-2所获得的数据表明，所有细胞系均有显著的生长抑制，但不同提取物对细胞的敏感性不同。线粒体活性的降低和凋亡的增加与所用制剂的凝集素含量呈剂量依赖性相关。
这些体外实验结果表明，儿童髓母细胞瘤细胞对欧洲槲寄生制剂有反应，通过凋亡导致细胞死亡，且这种生长抑制与所用制剂的凝集素含量相关。
