他莫昔芬联合塞来昔布对二甲基苯并蒽诱导的大鼠乳腺癌的化学预防作用

[Chemopreventive effect of tamoxifen combined with celecoxib on DMBA-induced breast cancer in rats].

作者信息

Kang Hua-Feng, Wang Xi-Jing, Liu Xiao-Xu, Dai Zhi-Jun, Xue Feng-Jie, Xue Xing-Huan

机构信息

Department of Oncologic Surgery, The Second Affiliated Hospital, School of Medcine, Xioan Jiaotong University, Xioan, Shaanxi, 710004, P. R. China.

出版信息

Ai Zheng. 2006 Nov;25(11):1346-50.

PMID:17094899

Abstract

BACKGROUND & OBJECTIVE: Breast cancer can be prevented partly by tamoxifen. Cyclooxygenase-2 (COX-2) is expressed in many kinds of tumors, and correlated to the occurrence and progress of tumors. This study was to evaluate the chemopreventive effect of tamoxifen combined with celecoxib, a COX-2 selective inhibitor, on 7,12-dimethylbenz anthracene (DMBA)-induced breast cancer in rats.

METHODS

DMBA was irrigated into the stomach of SD female rats to build breast cancer model. The rats were divided into 4 groups: control group, tamoxifen group, celecoxib group, and combination group; each group contained 30 rats. Tumor occurrence, latency period, number and volume of breast cancer were observed.

RESULTS

The tumor occurrence rates in tamoxifen group (48.15%, 13/27) and celecoxib group (50.00%, 14/28) were lower than that in control group (85.71%, 24/28), and higher than that in combination group (21.43%, 6/28). The latency periods of tamoxifen group [(97.54+/-1.85) days] and celecoxib group [(96.79+/-2.89) days] were longer than that of control group [(89.50+/-5.99) days], and shorter than that of combination group [(103.67+/-3.39) days]. The tumor numbers of tamoxifen group (1.77+/-0.73) and celecoxib group (1.71+/-0.61) were less than that of control group (3.50+/-1.62), and more than that of combination group (1.17+/-0.42). The tumor volumes of tamoxifen group [(1.78+/-0.71) cm(3)] and celecoxib group [(2.05+/-1.04) cm(3)] were smaller than that of control group [(6.42+/-3.96) cm(3)], and larger than that of combination group [(0.71+/-0.96) cm(3)]. All differences were significant (P<0.05, respectively).

CONCLUSION

Celecoxib and tamoxifen could effectively prevent the occurrence of DMBA-induced breast cancer in rats; the combination of them has better chemopreventive effect.

摘要

背景与目的

他莫昔芬可部分预防乳腺癌。环氧化酶-2（COX-2）在多种肿瘤中表达，与肿瘤的发生和进展相关。本研究旨在评估他莫昔芬联合COX-2选择性抑制剂塞来昔布对7,12-二甲基苯并蒽（DMBA）诱导的大鼠乳腺癌的化学预防作用。

方法

将DMBA灌胃至SD雌性大鼠胃内以建立乳腺癌模型。大鼠分为4组：对照组、他莫昔芬组、塞来昔布组和联合组；每组30只大鼠。观察肿瘤发生情况、潜伏期、乳腺癌的数量和体积。

结果

他莫昔芬组（48.15%，13/27）和塞来昔布组（50.00%，14/28）的肿瘤发生率低于对照组（85.71%，24/28），高于联合组（21.43%，6/28）。他莫昔芬组[（97.54±1.85）天]和塞来昔布组[（96.79±2.89）天]的潜伏期长于对照组[（89.50±5.99）天]，短于联合组[（103.67±3.39）天]。他莫昔芬组（1.77±0.73）和塞来昔布组（1.71±0.61）的肿瘤数量少于对照组（3.50±1.62），多于联合组（1.17±0.42）。他莫昔芬组[（1.78±0.71）cm³]和塞来昔布组[（2.05±1.04）cm³]的肿瘤体积小于对照组[（6.42±3.96）cm³]，大于联合组[（0.71±0.96）cm³]。所有差异均有统计学意义（P均<0.05）。