Komabayashi T, Yakata A, Izawa T, Suda K, Tsuboi M
Department of Physiology and Pharmacology, Tokyo College of Pharmacy, Japan.
Res Commun Chem Pathol Pharmacol. 1990 Dec;70(3):297-305.
We studied the role of sn-1, 2-diacylglycerol (DAG) accumulation in the potentiating effect of A23187 on carbachol (CCh)-stimulated amylase secretion from rat parotid acinar cells. A23187 (0.1 microM) linearly increased DAG accumulation with time for at least 30 min. At concentrations higher or lower than 0.1 microM, DAG levels increased for up to 20 min, but declined at 30 min. Dose-response curve for DAG accumulation induced by A23187 was similar to that for amylase secretion. A23187 augmented CCh-stimulated DAG accumulation and amylase secretion. These results suggest that DAG accumulation is involved in the potentiating effect of A23187 on CCh-stimulated amylase secretion.
我们研究了sn-1,2-二酰基甘油（DAG）积累在A23187增强卡巴胆碱（CCh）刺激大鼠腮腺腺泡细胞淀粉酶分泌效应中的作用。A23187（0.1微摩尔）使DAG积累随时间呈线性增加，至少持续30分钟。在高于或低于0.1微摩尔的浓度下，DAG水平在20分钟内升高，但在30分钟时下降。A23187诱导的DAG积累剂量反应曲线与淀粉酶分泌的曲线相似。A23187增强了CCh刺激的DAG积累和淀粉酶分泌。这些结果表明，DAG积累参与了A23187对CCh刺激的淀粉酶分泌的增强作用。
