Labriola L, Ferreira G Bomfim, Montor W R, Demasi M A A, Pimenta D C, Lojudice F H, Genzini T, Goldberg A C, Eliaschewitz F G, Sogayar M C
Department of Biochemistry, Chemistry Institute, University of Sao Paulo, Av. Prof. Lineu Prestes, 748, Bloco 9 Superior Sala 964, São Paulo 05508-900 SP, Brazil.
Mol Cell Endocrinol. 2007 Jan 29;264(1-2):16-27. doi: 10.1016/j.mce.2006.10.004. Epub 2006 Nov 13.
Ex vivo islet cell culture prior to transplantation appears as an attractive alternative for treatment of type 1 diabetes. Previous results from our laboratory have demonstrated beneficial effects of human prolactin (rhPRL) treatment on human islet primary cultures. In order to probe into the molecular events involved in the intracellular action of rhPRL in these cells, we set out to identify proteins with altered expression levels upon rhPRL cell treatment, using two-dimensional (2D) gel electrophoresis and mass spectrometry (MS). An average of 300 different protein spots were detected, 14 of which were modified upon rhPRL treatment (p<0.01), of which 12 were successfully identified using MS and grouped according to their biological functions. In conclusion, our study provides, for the first time, information about proteins that could be critically involved in PRL's action on human pancreatic islets, and facilitate identification of new and specific targets involved in islet cell function and proliferation.
移植前的离体胰岛细胞培养似乎是治疗1型糖尿病的一种有吸引力的替代方法。我们实验室之前的结果表明，人催乳素（rhPRL）治疗对人胰岛原代培养有有益作用。为了探究rhPRL在这些细胞内作用所涉及的分子事件，我们使用二维（2D）凝胶电泳和质谱（MS）来鉴定rhPRL细胞处理后表达水平发生改变的蛋白质。平均检测到300个不同的蛋白质斑点，其中14个在rhPRL处理后发生了改变（p<0.01），其中12个通过MS成功鉴定，并根据其生物学功能进行了分组。总之，我们的研究首次提供了可能与PRL对人胰岛作用密切相关的蛋白质信息，并有助于鉴定参与胰岛细胞功能和增殖的新的特定靶点。
