神经球蛋白和细胞球蛋白的过表达可保护人SH-SY5Y神经母细胞瘤细胞免受氧化应激诱导的细胞死亡。

Neuroglobin and cytoglobin overexpression protects human SH-SY5Y neuroblastoma cells against oxidative stress-induced cell death.

作者信息

Fordel Elke, Thijs Liesbet, Martinet Wim, Lenjou Marc, Laufs Tilmann, Van Bockstaele Dirk, Moens Luc, Dewilde Sylvia

机构信息

Department of Biomedical Sciences, University of Antwerp, Belgium.

出版信息

Neurosci Lett. 2006 Dec 20;410(2):146-51. doi: 10.1016/j.neulet.2006.09.027.

DOI:10.1016/j.neulet.2006.09.027

PMID:17095159

Abstract

Although reactive oxygen species (ROS) at physiological concentrations are required for normal cell function, excessive production of ROS is detrimental to cells. Neuroglobin and cytoglobin are two globins, whose functions are still a matter of debate. A potential role in the detoxification of ROS is suggested. The influence of neuroglobin and cytoglobin on cell death after oxidative stress in human neuroblastoma SH-SY5Y cells was evaluated. Exposure of SH-SY5Y cells to paraquat or H(2)O(2) resulted in a concentration- and time-dependent induction of apoptotic and necrotic cell death. H(2)O(2) was 16 times more potent to induce cell death as compared to paraquat. SH-SY5Y cells transfected with plasmid DNA containing the neuroglobin or cytoglobin sequence showed enhanced survival after exposure to 300 microM H(2)O(2) for 24h as compared to untransfected controls. This finding suggests that neuroglobin and cytoglobin protect SH-SY5Y cells against oxidative stress-induced cell death.

摘要

尽管生理浓度的活性氧（ROS）是正常细胞功能所必需的，但ROS的过量产生对细胞有害。神经球蛋白和细胞球蛋白是两种球蛋白，它们的功能仍存在争议。有人提出它们在ROS解毒中可能发挥作用。我们评估了神经球蛋白和细胞球蛋白对人神经母细胞瘤SH-SY5Y细胞氧化应激后细胞死亡的影响。将SH-SY5Y细胞暴露于百草枯或H₂O₂会导致凋亡和坏死性细胞死亡的浓度和时间依赖性诱导。与百草枯相比，H₂O₂诱导细胞死亡的效力高16倍。与未转染的对照相比，用含有神经球蛋白或细胞球蛋白序列的质粒DNA转染的SH-SY5Y细胞在暴露于300μM H₂O₂ 24小时后显示出更高的存活率。这一发现表明神经球蛋白和细胞球蛋白可保护SH-SY5Y细胞免受氧化应激诱导的细胞死亡。