Bobrov Nikita, Cavarga Ivan, Longauer Frantisek, Rybárová Silvia, Fedorocko Peter, Brezáni Peter, Miskovský Pavol, Mirossay Ladislav, Stubna Ján
P. J. Safárik University, Kosice, Slovakia.
Phytomedicine. 2007 Feb;14(2-3):172-8. doi: 10.1016/j.phymed.2006.09.017. Epub 2006 Nov 7.
Histomorphological changes in murine fibrosarcoma after photodynamic therapy (PDT) based on the natural photosensitizer hypericin were evaluated. C3H/DiSn mice were inoculated with fibrosarcoma G5:1:13 cells. When the tumour reached a volume of 40-80 mm(3) the mice were intraperitoneally injected with hypericin, either in a single dose (5 mg/kg; 1 or 6 h before laser irradiation) or two fractionated doses (2.5 mg/kg; 6 and 1 h before irradiation with laser light; 532 nm, 70 mW/cm(2), 168 J/cm(2)). All groups of PDT-treated animals with single and fractionated hypericin dosing presented primary vascular reactions including vascular dilatation, congestion, thrombosis and oedema. Two hours after PDT there were necrotic changes with small, rather focal appearance. One day after therapy the necrotic areas were enhanced, often affecting a complete superficial layer of tumour tissue. Necrotic areas were accompanied with inflammation and haemorrhages.
评估了基于天然光敏剂金丝桃素的光动力疗法（PDT）后小鼠纤维肉瘤的组织形态学变化。将纤维肉瘤G5:1:13细胞接种到C3H/DiSn小鼠体内。当肿瘤体积达到40 - 80立方毫米时，给小鼠腹腔注射金丝桃素，要么单次给药（5毫克/千克；激光照射前1或6小时），要么分两次给药（2.5毫克/千克；激光照射前6和1小时；532纳米，70毫瓦/平方厘米，168焦耳/平方厘米）。所有接受单次和分次金丝桃素给药的PDT治疗动物组均出现主要的血管反应，包括血管扩张、充血、血栓形成和水肿。PDT后两小时出现坏死变化，表现为小的、局灶性的。治疗后一天，坏死区域扩大，常常累及肿瘤组织的整个表层。坏死区域伴有炎症和出血。
