Khan Mohammed, Seppala Maisa, Zoupa Maria, Cobourne Martyn T
Department of Orthodontics, The Dental Institute of King's College London at Guy's, King's and St. Thomas', Guy's Hospital, London SE19RT, UK.
Gene Expr Patterns. 2007 Jan;7(3):239-43. doi: 10.1016/j.modgep.2006.10.001. Epub 2006 Oct 7.
Sonic hedgehog is a secreted protein important for many aspects of embryonic development. In the developing tooth, Shh expression is restricted to the epithelial compartment and plays an important role during both initiation and subsequent coronal morphogenesis. We have investigated the expression of Shh and constituent members of the signalling pathway during early development of the molar tooth root in the mouse and find the presence of transcripts in Hertwig's epithelial root sheath. These epithelial cells of the root sheath and the surrounding apical mesenchyme of the dental papilla and follicle also expressed the Shh receptor Ptc1, agonist Smo and Gli downstream transcriptional effectors; however, this response occurred over short range. In contrast, the Shh antagonists Hip1 and Gas1 were both expressed at a distance from these responding cells, in more peripheral regions of the developing root. Transcripts of the Skn acyl transferase lacked specific expression in early root structures.
音猬因子是一种分泌蛋白，对胚胎发育的许多方面都很重要。在发育中的牙齿中，Shh表达局限于上皮部分，在起始阶段和随后的冠部形态发生过程中都发挥着重要作用。我们研究了小鼠磨牙牙根早期发育过程中Shh及其信号通路组成成员的表达情况，发现赫特维希上皮根鞘中有转录本存在。根鞘的这些上皮细胞以及牙乳头和滤泡周围的顶端间充质也表达了Shh受体Ptc1、激动剂Smo和Gli下游转录效应物；然而，这种反应发生的范围较短。相比之下，Shh拮抗剂Hip1和Gas1都在远离这些反应细胞的位置表达，即在发育牙根的更外围区域。Skn酰基转移酶的转录本在早期牙根结构中缺乏特异性表达。
