Spelzini Federico, Konstantinovic Maja L, Guelinckx Isabelle, Verbist Godelieve, Verbeken Erik, De Ridder Dirk, Deprest Jan
Centre for Surgical Technologies, Faculty of Medicine, University Hospital Gasthuisberg, Katholieke Universiteit Leuven, Leuven, Belgium.
Gynecol Obstet Invest. 2007;63(3):155-62. doi: 10.1159/000096893. Epub 2006 Nov 8.
We compared host response, architectural integration and tensile strength of two different macroporous silk constructs to a polypropylene type I implant in a rat model for augmentation of primary fascial defect repair.
Animals were sacrificed on days 7, 14, 30 and 90 after implantation. The explants were evaluated macroscopically for infections, herniations and adhesions, mechanically for tensile strength, and histopathologically, to evaluate collagen deposition and inflammatory response.
The tensile strength of the explants showed a gradual increase for all materials. All implants uniformly shrank around one fifth by 90 days. In the silk implants, the inflammatory reaction showed a remarkable higher number of foreign body giant cells that characteristically spread from the periphery into implants. Collagen deposition was comparable for all the materials. In Silk a higher grade of neovascularisation was observed.
Silk explants expressed high tensiometric strength, which was associated with a marked fibrotic process. The silk implants induced a strong foreign body reaction accompanied by microscopic signs of architectural degradation at 90 days. Polypropylene explants showed a more moderate foreign body reaction without architectural disturbance.
在大鼠模型中，我们比较了两种不同的大孔丝素构建物与聚丙烯I型植入物在增强原发性筋膜缺损修复方面的宿主反应、结构整合和拉伸强度。
在植入后第7、14、30和90天处死动物。对外植体进行宏观评估以检查感染、疝形成和粘连情况，进行力学测试以评估拉伸强度，并进行组织病理学评估以评估胶原蛋白沉积和炎症反应。
所有材料的外植体拉伸强度均呈逐渐增加趋势。到90天时，所有植入物均均匀收缩了约五分之一。在丝素植入物中，炎症反应显示出大量异物巨细胞，这些细胞典型地从周边扩散到植入物内部。所有材料的胶原蛋白沉积情况相当。在丝素A中观察到更高程度的新生血管形成。
丝素外植体表现出较高的拉伸强度，这与明显的纤维化过程相关。丝素植入物在90天时引发了强烈的异物反应，并伴有微观结构降解的迹象。聚丙烯外植体表现出更适度的异物反应，且无结构紊乱。
