Liu Yan-Rong, Chen Shan-Shan, Chang Yan, Fu Jia-Yu, Zhang Le-Ping, Wang Hui, Li Ling-Di, Zhu Hong-Hu, Liu Gui-Lan, Lu Dao-Pei, Huang Xiao-Jun
Institute of Hematology, Peking University, Beijing 100044, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2006 Oct;14(5):853-7.
To evaluate the significance of FCM in minimal residual disease (MRD) detection, the immunophenotyping and leukemia-associated immunophenotypes (LAIP) of leukemia cells from 273 adult and 142 childhood patients with B lineage acute lymphoblastic leukemia (B-ALL) were detected by four to six antibody combinations of 4-color CD45/SSC gating multiparametric flow cytometry (FCM). The results showed that the B-ALL patients could be classified into 4 subtypes based on different expression CD34 and CD10: subtype I (CD34(+)/CD10(-)), subtype II (CD34(+)/CD10(+)), subtype III (CD34(-)/CD10(+)), subtype IV (CD34(-)/CD10(-)). The LAIP was observed in 100% and 92% patients of subtype I and subtype II, respectively, whereas only 79.2% in subtype III. The incidence of LAIP in total B-ALL cases was 90% by using the antibodies detected in this investigation. There was no significantce different for incidence of LAIP between adult and pediatric patients. LAIP was observed in 77.6% of patients by labeling only CD34/CD10/CD19/CD45 4-color antibody combination. It is concluded that in 90% of childhood and adult B-ALL patients LAIP can be found, which suits MRD detection by multiparameter flow cytometry.
为评估流式细胞术（FCM）在微小残留病（MRD）检测中的意义，采用四至六种抗体组合的四色CD45/SSC门控多参数流式细胞术（FCM）检测了273例成人和142例儿童B系急性淋巴细胞白血病（B-ALL）患者白血病细胞的免疫表型及白血病相关免疫表型（LAIP）。结果显示，B-ALL患者可根据CD34和CD10的不同表达分为4个亚型：I型（CD34(+)/CD10(-)）、II型（CD34(+)/CD10(+)）、III型（CD34(-)/CD10(+)）、IV型（CD34(-)/CD10(-)）。I型和II型患者中LAIP的观察率分别为100%和92%，而III型患者中仅为79.2%。采用本研究中检测的抗体，B-ALL总病例中LAIP的发生率为90%。成人和儿童患者LAIP的发生率无显著差异。仅标记CD34/CD10/CD19/CD45四色抗体组合时，77.6%的患者观察到LAIP。结论是，90%的儿童和成人B-ALL患者可检测到LAIP，适合采用多参数流式细胞术进行MRD检测。
