Qiu Yan, Zha Yi, Tan Ying-Xia, Zhang Yang-Pei
Beijing Red Cross Blood Center, Beijing 100088, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2006 Oct;14(5):1020-3.
The objective of study was to investigate the Rh antigen stability of mPEG-modified RBC. RBC membrane protein SDS-PAGE technology was used to analyze the combination of the mPEG modified RBC membrane protein with mPEG molecules; the RBC ghost coagulation test and 4 degrees C CPD-preserved modified RBC mixed with matched blood were used to observe the stability of RBC Rh antigen camouflaged by mPEG. The results showed that the blood groups of stored mPEG-modified RBC were kept consistency before or after simulating transfusion, i.e. mixture of modified RBC with matched bloods, while the plasma hemoglobin after simulating transfusion was not only within the normal range during the storage, but also less than that before simulating transfusion even after incubation at 37 degrees C. The electrophoresis pattern stained with iodine and Coomassie blue displayed the bands of mPEG combined with RBC membrane protein and the slow mobility of membrane protein. The hemagglutination of PEGylation RBC ghosts did not take place and mPEG still covered the antigen. In conclusion, mPEG-SPA can bind the erythrocyte with its extracted membrane protein in both ghosts and living erythrocytes.
本研究的目的是探究聚乙二醇（mPEG）修饰红细胞的Rh抗原稳定性。采用红细胞膜蛋白十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）技术分析mPEG修饰的红细胞膜蛋白与mPEG分子的结合情况；通过红细胞血影凝集试验以及4℃保存的修饰红细胞与配合血混合，观察mPEG掩盖的红细胞Rh抗原的稳定性。结果显示，模拟输血前后，储存的mPEG修饰红细胞的血型保持一致，即修饰红细胞与配合血混合后的血型一致，而模拟输血后的血浆血红蛋白不仅在储存期间处于正常范围内，甚至在37℃孵育后也低于模拟输血前。用碘和考马斯亮蓝染色的电泳图谱显示了mPEG与红细胞膜蛋白结合的条带以及膜蛋白的缓慢迁移。聚乙二醇化红细胞血影未发生凝集，mPEG仍覆盖着抗原。总之，mPEG-SPA能使红细胞与其提取的膜蛋白在血影和活红细胞中结合。
