Christiernin M
Department of Fiber and Polymer Technology, Royal Institute of Technology, KTH, 100 44 Stockholm, Sweden.
Plant Physiol Biochem. 2006 Nov-Dec;44(11-12):693-9. doi: 10.1016/j.plaphy.2006.10.015. Epub 2006 Oct 24.
The developing xylem in a Norway spruce (Picea abies) clone was investigated during a growth season and compared to lignin from sapwood of the same tree clone. Klason and acid-soluble lignin contents were determined as well as the carbohydrate monomer distribution and protein content. By analyzing lignin thioacidolysis products, it was shown that only guaiacyl units could be detected in the materials, and the relative amount of beta-O-4' bonds was assessed. Monomeric and selected dimeric lignin products were identified by mass spectrometry. The specimens were embedded and thin sections examined by microscopy to determine the state of cell differentiation in the samples. In the spring and early summer, growth was very rapid and the intention was to collect tissue in which exclusively the middle lamella/primary cell wall had begun to lignify. Combining data regarding Klason lignin, protein content and carbohydrate monomer distribution with microscopy, it was found that the developing xylem sample from mid-June contained lignin from exclusively middle lamella/primary wall. The Klason lignin content in the developing xylem during the growth season was 20%, 5% and 10% in April, June and August, respectively. Thioacidolysis showed that the lignin had more condensed structures than lignin from the reference Norway spruce clone wood. Mass spectrometry showed that the developing xylem specimens from June and August contained more lignin structures with end-groups than the reference sample. These results suggest that lignification in the cambial layer and early developing xylem may take place more in a bulk fashion during the summer.
在一个生长季节内对挪威云杉（Picea abies）无性系中正在发育的木质部进行了研究，并将其与同一树木无性系边材中的木质素进行了比较。测定了克拉森木质素和酸溶性木质素含量以及碳水化合物单体分布和蛋白质含量。通过分析木质素硫代酸解产物，结果表明在这些材料中仅能检测到愈创木基单元，并评估了β-O-4'键的相对含量。通过质谱鉴定了单体和选定的二聚体木质素产物。将标本包埋并通过显微镜检查薄切片以确定样品中细胞分化的状态。在春季和初夏，生长非常迅速，目的是收集仅中层薄片/初生细胞壁开始木质化的组织。将有关克拉森木质素、蛋白质含量和碳水化合物单体分布的数据与显微镜观察相结合，发现6月中旬发育中的木质部样品仅含有来自中层薄片/初生壁的木质素。在生长季节，4月、6月和8月发育中的木质部中克拉森木质素含量分别为20%、5%和10%。硫代酸解表明，该木质素比来自参考挪威云杉无性系木材的木质素具有更多的缩合结构。质谱分析表明，6月和8月发育中的木质部标本比参考样品含有更多带有端基的木质素结构。这些结果表明，形成层和早期发育的木质部中的木质化在夏季可能更多地以整体方式发生。
