Kukkola Eija M, Koutaniemi Sanna, Pöllänen Eija, Gustafsson Mikaela, Karhunen Pirkko, Lundell Taina K, Saranpää Pekka, Kilpeläinen Ilkka, Teeri Teemu H, Fagerstedt Kurt V
Department of Biosciences, Helsinki University, PO Box 56, 00014, Helsinki, Finland.
Planta. 2004 Jan;218(3):497-500. doi: 10.1007/s00425-003-1107-3. Epub 2003 Sep 12.
A specific condensed lignin substructure, dibenzodioxocin, was immunolocalized in differentiating cell walls of Norway spruce ( Picea abies (L.) H. Karsten) and silver birch ( Betula pendula Roth) xylem. A fluorescent probe, Alexa 488 was used as a marker on the dibenzodioxocin-specific secondary antibody. For the detection of this lignin substructure, 25-microm cross-sections of xylem were viewed with a confocal laser-scanning microscope with fluorescein isothiocyanate fluorescence filters. In mature cells, fluorescence was detected in the S3 layer of the secondary wall in both tree species, but it was more intense in Norway spruce than in silver birch. In silver birch most of the signal was detected in vessel walls and less in fiber cell walls. In very young tracheids of Norway spruce and vessels and fibers of silver birch, where secondary cell wall layers were not yet formed, the presence of the dibenzodioxocin structure could not be shown.
一种特定的缩合木质素亚结构——二苯并二恶英，在挪威云杉（Picea abies (L.) H. Karsten）和银桦（Betula pendula Roth）木质部分化细胞壁中被免疫定位。一种荧光探针Alexa 488被用作二苯并二恶英特异性二抗上的标记物。为了检测这种木质素亚结构，使用配备异硫氰酸荧光素荧光滤光片的共聚焦激光扫描显微镜观察25微米厚的木质部横截面。在成熟细胞中，两种树种的次生壁S3层均检测到荧光，但挪威云杉中的荧光比银桦中的更强。在银桦中，大部分信号在导管壁中检测到，在纤维细胞壁中较少。在挪威云杉非常幼嫩的管胞以及银桦的导管和纤维中，尚未形成次生细胞壁层，未显示出二苯并二恶英结构的存在。
