利用重组大肠杆菌以干酪乳清为底物和诱导剂生产青霉素酰化酶。

Production of penicillin acylase by a recombinant Escherichia coli using cheese whey as substrate and inducer.

作者信息

De León-Rodríguez Antonio, Rivera-Pastrana Dulce, Medina-Rivero Emilio, Flores-Flores José Luis, Estrada-Baltazar Alejandro, Ordóñez-Acevedo Leandro G, de la Rosa Ana P Barba

机构信息

División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, Apartado Postal 3-74 Tangamanga, 78231 San Luis Potosí, S.L.P., Mexico.

出版信息

Biomol Eng. 2006 Dec;23(6):299-305. doi: 10.1016/j.bioeng.2006.09.003. Epub 2006 Nov 9.

DOI:10.1016/j.bioeng.2006.09.003

PMID:17097344

Abstract

Cheese whey (CW) is the major subproduct from cheese manufacturing and it is considered as a waste pollutant since its high content of lactose. In this work a fermentation process for the production of penicillin acylase (PA) by a recombinant Escherichia coli and using CW as unique carbon source and inducer was developed. A design factorial 3(2) was used to evaluate the influence of independent variables (dissolved oxygen and CW concentration) on the ability of E. coli W3110/pPA102 to produce PA. Maximum specific PA activity of 781 U g(-1) was attained at 5 g L(-1) of CW and 3% dissolved oxygen. The results showed that CW can be used successfully as unique carbon source and inducer for the production of recombinant proteins using constructions driven by the lac promoter and this way reducing the discharges of that pollutant to the environment.

摘要

奶酪乳清（CW）是奶酪制造过程中的主要副产品，由于其乳糖含量高，被视为一种废弃物污染物。在这项工作中，开发了一种利用重组大肠杆菌生产青霉素酰化酶（PA）的发酵工艺，该工艺使用CW作为唯一碳源和诱导剂。采用3(2)析因设计来评估自变量（溶解氧和CW浓度）对大肠杆菌W3110/pPA102生产PA能力的影响。在CW浓度为5 g L(-1)和溶解氧为3%时，获得了781 U g(-1)的最大比PA活性。结果表明，CW可以成功用作唯一碳源和诱导剂，用于生产由乳糖启动子驱动构建体的重组蛋白，从而减少该污染物向环境中的排放。