Vera-Sempere F J, Rubio L, Felipe-Ponce V, Garcia A, Sanahuja M J, Zamora I, Ramos D, Beneyto I, Sánchez-Plumed J
Service of Pathology, Laboratory of Molecular Pathology, University Medical School, Valencia, Spain.
Transplant Proc. 2006 Oct;38(8):2378-81. doi: 10.1016/j.transproceed.2006.08.049.
BK virus (BKV) reactivation in immunocompromised kidney transplant patients can produce a tubulointerstitial nephropathy (BKVN). Molecular tools that test for DNA-BKV provide early detection and assist in management, but some aspects of the pathogenesis of this infection, such as donor causality, remain unclear.
Between November 2004 and January 2006, 55 Spanish kidney donors were studied for BK infection. A quantitative PCR assay was performed on urine and serum to detect BKV. To determine the origin of the viral infection, a transcription control region of the BK polymorphism sequence was designed to identify the viral subtype.
Fifteen of 55 (27%) donors were BK-PCR positive: 13 in urine and 2 in serum and urine. Moreover, monitoring of recipient pairs detected BK-PCR positivity in 14 of 73 recipients. We studied eight BK-PCR positive recipients (corresponding to four pairs) and their respective donors. The same viral genome was observed in the four pairs, namely, the A250-1-a, WW-like, AS, and JL genotypes. Interestingly, one of the four pairs showed the donor and the two recipients to display exactly the same JL genotype.
On the basis of our preliminary results analyzing the molecular fingerprints of donor and recipient pairs, we have presented new data implicating the donor, in at least some cases, as the source of BK infection.
免疫功能低下的肾移植患者中BK病毒（BKV）再激活可导致肾小管间质性肾病（BKVN）。检测DNA-BKV的分子工具可实现早期检测并有助于管理，但这种感染的发病机制的某些方面，如供体因果关系，仍不清楚。
2004年11月至2006年1月，对55名西班牙肾供体进行BK感染研究。对尿液和血清进行定量PCR检测以检测BKV。为确定病毒感染的来源，设计了BK多态性序列的转录控制区以鉴定病毒亚型。
55名供体中有15名（27%）BK-PCR呈阳性：13名尿液阳性，2名血清和尿液阳性。此外，对受者配对的监测发现73名受者中有14名BK-PCR呈阳性。我们研究了8名BK-PCR阳性受者（对应4对）及其各自的供体。在这4对中观察到相同的病毒基因组，即A250-1-a、WW样、AS和JL基因型。有趣的是，4对中的一对显示供体和两名受者表现出完全相同的JL基因型。
基于我们对供体和受者配对分子指纹分析的初步结果，我们提供了新的数据，表明至少在某些情况下，供体是BK感染的来源。
