Barbosa Taylor, Zavala Guillermo, Cheng Sunny, Villegas Pedro
Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, GA 30602-4875, USA.
Virus Res. 2007 Mar;124(1-2):68-77. doi: 10.1016/j.virusres.2006.10.002. Epub 2006 Nov 13.
Reticuloendotheliosis virus (REV) causes runting, high mortality, immunosuppression, and chronic neoplasia associated with T and/or B cell lymphomas in a variety of domestic and wild birds, including Attwater's prairie chickens (APC) (Tympanuchus cupido attwateri). The complete proviral sequence of a recent REV isolate from APC (REV APC-566) was determined. This virus was isolated from an APC maintained in captivity in a reproduction program intended to avoid its extinction. REV APC-566 was determined to be oncogenic in Japanese quail (Coturnix coturnix japonica), chickens (Gallus gallus) and turkeys (Meleagris gallopavo). Immune responses against bacteria and viruses were significantly reduced in turkeys infected with REV APC-566. The proviral genome is 8286 nucleotides in length and exhibits a genetic organization characteristic of replication-competent gammaretroviruses. The REV APC-566 provirus contains two identical long terminal repeats (LTR) and a complete set of genes including gag, gag-pol and env. As previously reported, alignments with other REV sequences showed high similarity with sequences found in the gag and pol genes from other REVs. The REV APC-566 env gene showed high nucleotide sequence homology with REV sequences inserted in fowl poxvirus (99.8%), and with spleen necrosis virus (SNV) (95.1%). Sequences coding for a previously reported immunosuppressive peptide contained in the transmembrane region of the env gene are well conserved among all REV sequences analyzed. The LTR was the most divergent region, exhibiting various deletions and insertions. REV APC-566 has a unique insertion of 23 bp in U3 and shares deletions of 19 and 5 bp with chicken syncytial virus and REV inserts in fowlpox virus.
网状内皮组织增生症病毒（REV）可导致多种家养和野生鸟类出现发育迟缓、高死亡率、免疫抑制以及与T和/或B细胞淋巴瘤相关的慢性肿瘤，包括阿特沃特草原鸡（APC）（Tympanuchus cupido attwateri）。确定了从APC分离出的一株近期REV毒株（REV APC - 566）的完整前病毒序列。该病毒是从一个繁殖项目中圈养的一只APC分离出来的，该项目旨在避免其灭绝。REV APC - 566被确定在日本鹌鹑（Coturnix coturnix japonica）、鸡（Gallus gallus）和火鸡（Meleagris gallopavo）中具有致癌性。感染REV APC - 566的火鸡对细菌和病毒的免疫反应显著降低。前病毒基因组长度为8286个核苷酸，呈现出具有复制能力的γ逆转录病毒的遗传组织特征。REV APC - 566前病毒包含两个相同的长末端重复序列（LTR）以及一套完整的基因，包括gag、gag - pol和env。如先前报道，与其他REV序列比对显示，其与其他REV的gag和pol基因中的序列具有高度相似性。REV APC - 566的env基因与插入禽痘病毒中的REV序列（99.8%）以及脾坏死病毒（SNV）（95.1%）具有高度核苷酸序列同源性。在所有分析的REV序列中，env基因跨膜区域中编码先前报道的免疫抑制肽的序列高度保守。LTR是差异最大的区域，存在各种缺失和插入。REV APC - 566在U3区域有一个23 bp的独特插入，并且与鸡合胞体病毒以及插入禽痘病毒的REV共享19 bp和5 bp的缺失。
