Li Xiao-Hui, Xu Han-Kun, Mao Da-Gan, Ma Da-Jun, Chen Peng, Yang Li-Guo
Research Institute of Animal Breeding & Reproduction, Nanjing Agricultural University, Nanjing 210095, China.
Yi Chuan. 2006 Nov;28(11):1376-82. doi: 10.1360/yc-006-1376.
Excitability, activity and exploration behavior of puppies in a novel open-field were tested in a total of 204 two-month-old German shepherd dog, labrador retriever or English springer spaniel puppies. The polymorphisms of monoamine oxidase B gene (MAOB) were detected by PCR-RFLP. Statistics analysis indicated that genotype and allele frequencies of the polymorphisms were significantly different among three breeds (P < 0.01). With GLM analysis of SAS software, association analysis was conducted between MAOB gene polymorphisms and locomotion and vocalization behavior parameters in the open-field test. The results showed that MAOB gene polymorphisms had a significant effect on walking time, squares crossed, lying time, the times of standing up against walls(P < 0.01 or P < 0.05) and were associated with the times of posture change (P=0.064). Walking time and squares crossed were higher in TT genotype puppies than those in TC and CC puppies (P < 0.05) and the times of posture change and standing up against walls were also higher than those in CC (P < 0.05). In addition, lying time in CC genotype puppies were higher than that in TT (P < 0.05). MAOB had a positive effect on walking time, lying time, squares crossed, the times of posture change, the times of standing up against walls in the three dog breeds that was highly statistically significant (P < 0.01 or P < 0.05). Our results imply that MAOB gene significantly affects the excitability, activity and exploration behavior of puppies in open-field test and TT genotype has favorable effects in these behavior traits.
在总共204只两个月大的德国牧羊犬、拉布拉多猎犬或英国激飞猎犬幼犬中，测试了它们在新型旷场中的兴奋性、活动和探索行为。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）检测单胺氧化酶B基因（MAOB）的多态性。统计分析表明，三个品种之间多态性的基因型和等位基因频率存在显著差异（P<0.01）。使用SAS软件的广义线性模型（GLM）分析，在旷场试验中对MAOB基因多态性与运动和发声行为参数进行关联分析。结果表明，MAOB基因多态性对行走时间、穿越格数、躺卧时间、靠墙站立次数有显著影响（P<0.01或P<0.05），并与姿势变化次数相关（P=0.064）。TT基因型幼犬的行走时间和穿越格数高于TC和CC基因型幼犬（P<0.05），姿势变化次数和靠墙站立次数也高于CC基因型幼犬（P<0.05）。此外，CC基因型幼犬的躺卧时间高于TT基因型幼犬（P<0.05）。MAOB对三个犬种的行走时间、躺卧时间、穿越格数、姿势变化次数、靠墙站立次数有积极影响，具有高度统计学意义（P<0.01或P<0.05）。我们的结果表明，MAOB基因在旷场试验中显著影响幼犬的兴奋性、活动和探索行为，TT基因型在这些行为特征方面具有有利影响。
