Lin Fei, Wan Su-Qin, Cheng Li-Guo, Lü Hai-Yan, Li Guang-Jun, Zhang Yuan-Ming
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.
Yi Chuan. 2006 Nov;28(11):1407-10. doi: 10.1360/yc-006-1407.
For mapping QTLs, phenotypes of the traits in segregating population derived from the cross between two isogenic lines of the targeted QTL may reflect its genotype if the effect of the QTL is relatively large. In order to map the QTL, it is necessary to use a large sample under the high density of markers around the QTL. However, it increases experimental costs. In order to save the costs, it is possible to map the QTL using the sub-population that consists of plants with homogenous recessive. In this paper, the sub-population was used to estimate the recombination fraction between the marker and the QTL, and its standard error for F2, backcross (BC), double haploid (DH) and recombinant inbred lines (RIL) populations, respectively. The results from Monte Carlo simulation showed that the estimation of recombination fraction based on the sub-population is consistent with that obtained from the full population, and the precision of the former is same as that of the later under the same sample size.
对于数量性状基因座（QTL）定位而言，如果目标QTL的效应相对较大，那么由两个等基因系杂交产生的分离群体中该性状的表型可能反映其基因型。为了定位QTL，有必要在QTL周围标记高密度的情况下使用大样本。然而，这会增加实验成本。为了节省成本，可以使用由纯合隐性植株组成的亚群体来定位QTL。本文分别利用该亚群体估计了F2、回交（BC）、双单倍体（DH）和重组自交系（RIL）群体中标记与QTL之间的重组率及其标准误。蒙特卡罗模拟结果表明，基于亚群体的重组率估计与全群体获得的估计一致，并且在相同样本量下，前者的精度与后者相同。
