Luthman M, Bremme K, Jónsdóttir I, Hall K, Roos P, Werner S
Department of Endocrinology, Karolinska Hospital Stockholm, Sweden.
Gynecol Obstet Invest. 1991;31(2):67-73. doi: 10.1159/000293105.
Growth hormone (GH), placental lactogen (PL), prolactin (PRL), insulin-like growth factor I (IGF-I) and IGF binding protein-1 (IGFBP-1) were determined in serum by radioimmunoassays (RIAs) in 12 women during pregnancy. GH and PL were analyzed by two monoclonal antibodies (Mab 3 and Mab 1) raised against pituitary GH. Serum IGFBP-1 had reached maximum levels at midpregnancy while PRL, PL and IGF-I increased continuously during pregnancy. Mab 1, which cross-reacts with PL, measured consistently higher levels of PL in serum than a commercial PL RIA (p less than 0.01) due to interference of cross-reacting serum proteins in the Mab 1 RIA. The GH-specific Mab 3 showed decreasing GH levels in unfractionated serum throughout gestation, but detected GH-immunoreactive proteins of approximately 40-200 kD after molecular sieve chromatography of pooled serum from late pregnancy. It is suggested that the formation of GH complexes of large molecular mass account for the successive disappearance of monomeric GH during pregnancy.
采用放射免疫分析法（RIA）对12名孕妇血清中的生长激素（GH）、胎盘催乳素（PL）、催乳素（PRL）、胰岛素样生长因子I（IGF-I）和IGF结合蛋白-1（IGFBP-1）进行了测定。GH和PL采用针对垂体GH产生的两种单克隆抗体（Mab 3和Mab 1）进行分析。血清IGFBP-1在妊娠中期达到最高水平，而PRL、PL和IGF-I在孕期持续升高。由于Mab 1放射免疫分析中交叉反应血清蛋白的干扰，与PL发生交叉反应的Mab 1所检测的血清中PL水平始终高于商业PL放射免疫分析（p<0.01）。GH特异性的Mab 3显示，在整个妊娠期未分级血清中GH水平下降，但在对妊娠晚期混合血清进行分子筛层析后，检测到分子量约为40 - 200 kD的GH免疫反应性蛋白。提示妊娠期间大分子质量GH复合物的形成是导致单体GH相继消失的原因。
