Michael Kathrin, Böttcher Sindy, Klupp Barbara G, Karger Axel, Mettenleiter Thomas C
Institute of Molecular Biology, Friedrich-Loeffler-Institut, Boddenblick 5A, 17493 Greifswald-Insel Riems, Germany.
J Gen Virol. 2006 Dec;87(Pt 12):3503-3507. doi: 10.1099/vir.0.82168-0.
Proteins of the virion tegument of alphaherpesviruses are involved in protein-protein interactions, which play important roles in virus morphogenesis. Seven single-gene deletion mutants of Pseudorabies virus were analysed for alterations in the overall composition of the virion beyond the loss of the targeted protein. The UL36 protein (pUL36) was present in equal amounts in wild-type virions and mutants lacking pUL21, pUL49, pUL51, pUS3 or pUS8. Virions lacking pUL11 or pUL16 incorporated less full-length pUL36 than wild-type particles, but contained increased amounts of an N-terminal fragment of pUL36 that is present only in traces in wild-type virus and the other mutants.
α疱疹病毒的病毒体被膜蛋白参与蛋白质-蛋白质相互作用，这在病毒形态发生中起重要作用。分析了伪狂犬病病毒的七个单基因缺失突变体，以研究除目标蛋白缺失外病毒体整体组成的变化。UL36蛋白（pUL36）在野生型病毒体和缺乏pUL21、pUL49、pUL51、pUS3或pUS8的突变体中含量相等。缺乏pUL11或pUL16的病毒体比野生型颗粒掺入的全长pUL36更少，但含有增加量的pUL36 N端片段，该片段在野生型病毒和其他突变体中仅微量存在。
