Tobler Kurt, Favrot Claude, Nespeca Gilles, Ackermann Mathias
Institute for Virology, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 266a, CH-8057 Zurich, Switzerland.
Clinic for Small Animal Internal Medicine, Dermatology Unit, University of Zurich, Winterthurerstrasse 266a, CH-8057 Zurich, Switzerland.
J Gen Virol. 2006 Dec;87(Pt 12):3551-3557. doi: 10.1099/vir.0.82305-0.
Epidermodysplasia verruciformis (EV) is a rare human genetic predisposition to develop flat warts, some of which subsequently undergo cancer transformation. Some human papillomaviruses (HPVs), i.e. HPV 5 and 8, have been associated with cancer development as a sequela of EV. As similar diseases have been observed in dogs, it was hypothesized that unknown canine papillomaviruses (CPVs) may exist and that they may be present in cases of canine EV. Consequently, DNA was extracted from a malignant lesion of a dog with EV and circular DNA was amplified by multiple-primed rolling-circle amplification (RCA). Indeed, sequence determination and analysis of the RCA-amplified and cloned DNA from a malignant canine EV lesion resulted in the detection and primary description of a third CPV (CPV3). Typical papillomavirus genes were identified, with deduced amino acid similarities ranging from 20 to 57 % for E1, E2, E6, E7, L1 and L2, respectively. According to the sequence of the L1 gene, which is used for papillomavirus classification, the new isolate meets the majority of criteria needed to declare detection of a novel genus among the papillomaviruses. Thus, CPV3 may represent the prototype of this novel genus. As the novel virus was found in a dog in association with lesions reminiscent of human EV, it should be interesting to test in the future whether this condition can be reproduced in experimental animals. If such were the case, a new model for EV could be established.
疣状表皮发育不良（EV）是一种罕见的人类遗传易感性疾病，易患扁平疣，其中一些扁平疣随后会发生癌变。一些人乳头瘤病毒（HPV），即HPV 5和8，与EV继发的癌症发展有关。由于在犬类中观察到了类似疾病，因此推测可能存在未知的犬乳头瘤病毒（CPV），并且它们可能存在于犬类EV病例中。因此，从一只患有EV的犬的恶性病变中提取DNA，并通过多引物滚环扩增（RCA）扩增环状DNA。事实上，对来自恶性犬类EV病变的RCA扩增和克隆DNA进行序列测定和分析，结果检测并初步描述了第三种CPV（CPV3）。鉴定出了典型的乳头瘤病毒基因，E1、E2、E6、E7、L1和L2的推导氨基酸相似性分别为20%至57%。根据用于乳头瘤病毒分类的L1基因序列，新分离株符合在乳头瘤病毒中宣布检测到新属所需的大多数标准。因此，CPV3可能代表这个新属的原型。由于在一只犬中发现了这种新型病毒，且其病变类似于人类EV，未来测试这种情况是否能在实验动物中重现将很有趣。如果是这样，就可以建立一个新的EV模型。
