School of Veterinary Medicine, National Taiwan University, Taipei 10617, Taiwan.
Graduate Institute of Molecular and Comparative Pathobiology, School of Veterinary Medicine, National Taiwan University, Taipei 106, Taiwan.
Viruses. 2020 Feb 3;12(2):170. doi: 10.3390/v12020170.
Papillomavirus (PV) mainly infects the squamous epithelium and may potentially lead to benign or even malignant cutaneous lesions. However, the malignant transforming ability has been identified in several types of PVs. In humans, papillomavirus (HPV) type 16 and 18 are the most prevalent causative agents of cervical cancer. Therefore, vaccines are being developed to protect against these types. For dogs, there have been limited investigations into the association of different canine papillomavirus (CPV) genotypes with malignant lesions. Understanding the high-risk CPV genotype(s) responsible for these malignant lesions would contribute to the development of interventions for preventing CPV-induced carcinomas. In the present study, a retrospective cohort of 102 pathologically confirmed papillomas and 212 squamous cell carcinomas (SCCs) were included. The viral genome and antigens in the formalin-fixed paraffin-embedded (FFPE) tissues were detected using PCR targeting pan PV E1 and COPV L1 genes and by immunohistochemistry staining (IHC), respectively. PVs were successfully detected from 11 FFPE cutaneous tissues and four oral tissues using pan PV E1- and COPV L1-based PCR, respectively. After sequencing, CPV 1, CPV 2, and CPV 6 were detected in the benign lesions using PCR and were confirmed through IHC. While CPV 9 and CPV 15 were first detected in the SCCs of dogs, CPV 16 was most often detected in SCC specimens. The association and confirmative demonstration of viral genes and intralesional antigens of CPV 9, CPV 15, and CPV 16 in SCCs highlight the potential risk of these genotypes of CPVs in malignant transformation.
人乳头瘤病毒（PV）主要感染鳞状上皮，可能导致良性甚至恶性皮肤病变。然而，几种类型的 PV 已被证实具有恶性转化能力。在人类中，乳头瘤病毒（HPV）16 型和 18 型是宫颈癌最常见的致病因素。因此，正在开发疫苗来预防这些类型的感染。对于狗，对不同犬乳头瘤病毒（CPV）基因型与恶性病变的相关性的研究有限。了解导致这些恶性病变的高危 CPV 基因型将有助于开发预防 CPV 诱导的癌的干预措施。在本研究中，回顾性纳入了 102 例经病理证实的乳头瘤和 212 例鳞状细胞癌（SCC）。使用针对泛 PV E1 和 COPV L1 基因的 PCR 以及免疫组织化学染色（IHC）分别检测福尔马林固定石蜡包埋（FFPE）组织中的病毒基因组和抗原。使用基于泛 PV E1 和 COPV L1 的 PCR 分别从 11 份 FFPE 皮肤组织和 4 份口腔组织中成功检测到 PV。测序后，通过 PCR 在良性病变中检测到 CPV 1、CPV 2 和 CPV 6，并通过 IHC 确认。而 CPV 9 和 CPV 15 首次在犬 SCC 中检测到，CPV 16 则最常在 SCC 标本中检测到。CPV 9、CPV 15 和 CPV 16 的病毒基因和肿瘤内抗原在 SCC 中的关联和确认表明这些 CPV 基因型在恶性转化中的潜在风险。
