Zakhlebnaia O D, Laukner I V
Lab Delo. 1991(2):62-3.
Stabilizing effects of 5 percent aqueous polyvinylpyrrolidone (PVP) solution and sucrose-agar-gelatin medium (SAG) were examined in lyophilization of 4 Brucella types. The number of viable cells immediately after lyophilization in PVP has made up 48.1 percent on an average; this value was different with different Brucella species: 70.9 percent with B. abortus, 50.4 percent with B. melitensis, 41.2 percent with B. rangiferi, 30.2 percent with B. suis. Only 27.5 percent of cells were viable after Brucella lyophilization in SAG medium. Gradual death of lyophilized cells was observed in the course of strain storage at 5 degrees C. After two-year storage the number of viable cells after lyophilization in PVP was 41.8, after three-year storage 35.1 percent. The number of viable cells after SAG lyophilization was still lower. Biological characteristics of the strains lyophilized in both media corresponded to the initial ones over the three-year follow-up.
研究了5%聚乙烯吡咯烷酮（PVP）水溶液和蔗糖 - 琼脂 - 明胶培养基（SAG）在4种布鲁氏菌冻干过程中的稳定作用。冻干后立即在PVP中存活的细胞数量平均占48.1%；该数值因布鲁氏菌种类不同而有所差异：流产布鲁氏菌为70.9%，羊种布鲁氏菌为50.4%，驯鹿种布鲁氏菌为41.2%，猪种布鲁氏菌为30.2%。在SAG培养基中冻干布鲁氏菌后，仅有27.5%的细胞存活。在5℃菌株保存过程中，观察到冻干细胞逐渐死亡。保存两年后，在PVP中冻干后存活的细胞数量为41.8%，保存三年后为35.1%。SAG冻干后存活细胞数量更低。在三年的随访中，两种培养基冻干菌株的生物学特性均与初始特性相符。
