Kashuba V I, Skripkina I Ia, Saraev D V, Gordiiuk V V, Vinnitskaia A B, Tsyba L A, Pogrebnoĭ P V, Blinov V M, Zabarovskiĭ E R, Ryndich A V
Ukr Biokhim Zh (1999). 2006 Mar-Apr;78(2):113-20.
The investigation of the cancer-associated structural and epigenetic changes in cell genome is a major approach for understanding mechanisms of cancerogenesis. To investigate these genome changes, novel technique of microarrays comprising NotI-linking genome clones was developed. Twenty eight samples from patients with cervical cancer were analyzed using NotI microarrays of human chromosome 3. Deletions, amplifications and methylation were detected for 109 out of 182 NotI clones with different frequency. Notably, 17 NotI-linking clones showed genomic changes in more than 35% of tumor samples investigated, which suggests involvement of genes associated with these clones in development of cervical cancer.
对细胞基因组中与癌症相关的结构和表观遗传变化进行研究是理解癌症发生机制的主要途径。为了研究这些基因组变化，开发了包含NotI连接基因组克隆的微阵列新技术。使用人类3号染色体的NotI微阵列对28例宫颈癌患者的样本进行了分析。在182个NotI克隆中的109个中检测到了不同频率的缺失、扩增和甲基化。值得注意的是，17个NotI连接克隆在超过35%的研究肿瘤样本中显示出基因组变化，这表明与这些克隆相关的基因参与了宫颈癌的发生发展。
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