结直肠癌中人类3号染色体基因的表观遗传和遗传紊乱筛查

Screening of epigenetic and genetic disturbances of human chromosome 3 genes in colorectal cancer.

作者信息

Gerashchenko G V, Gordiyuk V V, Skrypkina I Ya, Kvasha S M, Kolesnik O O, Ugryn D D, Pavlova T V, Zabarovsky E R, Rynditch A V, Kashuba V I

机构信息

Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv.

出版信息

Ukr Biokhim Zh (1999). 2009 Jul-Aug;81(4):81-7.

PMID:20387637

Abstract

DNA microarray technology comprising NotI-linking clones was used in a large-scale study of genetic and epigenetic changes in colorectal cancer. Analysis of samples from 24 patients revealed methylation, deletions, and amplifications in 137 of 181 NotI clones. For 27 genes/loci, these changes occurred in more than 30% of the tumor samples, suggesting that these genes are involved in the development of colorectal cancer. An analysis of the methylation status of CpG island of the ITGA9 gene/loci by bisulfite sequencing confirmed the NotI microarray data on the gene/loci methylation in colorectal cancer. Aberrations in 19 genes/loci were unknown previously. Their characterization may help ascertain the mechanisms responsible for colorectal cancer development and identify novel diagnostic and prognostic markers.

摘要

包含NotI连接克隆的DNA微阵列技术被用于一项关于结直肠癌基因和表观遗传变化的大规模研究。对24例患者样本的分析显示，在181个NotI克隆中有137个存在甲基化、缺失和扩增。对于27个基因/基因座，这些变化发生在超过30%的肿瘤样本中，表明这些基因参与了结直肠癌的发生发展。通过亚硫酸氢盐测序对ITGA9基因/基因座的CpG岛甲基化状态进行分析，证实了NotI微阵列关于结直肠癌中该基因/基因座甲基化的数据。19个基因/基因座的异常情况此前未知。对它们的表征可能有助于确定结直肠癌发生发展的机制，并识别新的诊断和预后标志物。