Platonova T M, Chernyshenko T M, Sokolovs'ka L I
Ukr Biokhim Zh (1999). 2006 May-Jun;78(3):124-9.
The inhibitory effect of D- and DD-fragments and their mixture on plasma blood coagulation process under the action of thrombin has been studied. The significant increase of total efficiency of the inhibitory action has been shown. The inhibitors have influence on a lag-phase and lateral association of protofibrills of fibrin polymerization carried out by turbidimetry method in the model systems in vitro. The concentration of fragments which are close to parameters of fibrinogen/fibrin degradation products in pathological conditions was used. It is shown, that the covalent cross-link of D-dimer gamma-gamma-chains affects the inhibitory features and mechanism of interaction with fibrin monomer molecules. D-fragments inhibit the both phases of polymerization, whereas DD-fragments effectively inhibit the lateral association of protofibrill.
研究了D片段和DD片段及其混合物在凝血酶作用下对血浆血液凝固过程的抑制作用。结果表明抑制作用的总效率显著提高。通过体外模型系统中的比浊法，这些抑制剂对纤维蛋白原聚合的原纤维的滞后阶段和横向缔合有影响。使用了接近病理条件下纤维蛋白原/纤维蛋白降解产物参数的片段浓度。结果表明，D-二聚体γ-γ链的共价交联影响其抑制特性以及与纤维蛋白单体分子的相互作用机制。D片段抑制聚合的两个阶段，而DD片段有效抑制原纤维的横向缔合。
