Van Gool J, Van Tiel D, Doenhoff M J, Van Vugt H
Laboratory of Experimental Medicine, Academic Medical Centre, University of Amsterdam, The Netherlands.
Parasitology. 1991 Feb;102 Pt 1:49-56. doi: 10.1017/s0031182000060339.
A new model for the study of granuloma formation in the liver is described. Rats received an injection of 20,000 Schistosoma mansoni eggs in the portal vein and granuloma formation was evaluated at 3, 5 and 7 weeks post-injection. Liver collagen was estimated at the same time and serum procollagen III peptide, a marker of collagenesis, weekly. With this model, wherein the number of S. mansoni eggs and the time of injury are standardized, the effect of high levels of acute phase proteins especially alpha 2-macroglobulin on granuloma formation was studied. It appeared that in rats with high levels of alpha 2-macroglobulin the mean size of granulomas was significantly greater at 3 and 5 weeks compared with controls. In both groups an increase in liver collagen was observed during this period, reaching a peak at 5 weeks in the acute phase group. This model facilitates the study of the effects of S. mansoni eggs on granuloma formation.
本文描述了一种用于研究肝脏肉芽肿形成的新模型。给大鼠门静脉注射20,000个曼氏血吸虫卵，并在注射后3、5和7周评估肉芽肿形成情况。同时估计肝脏胶原蛋白含量，并每周检测血清前胶原III肽（一种胶原生成的标志物）。在这个模型中，曼氏血吸虫卵的数量和损伤时间是标准化的，研究了高水平急性期蛋白尤其是α2-巨球蛋白对肉芽肿形成的影响。结果显示，与对照组相比，α2-巨球蛋白水平高的大鼠在3周和5周时肉芽肿的平均大小明显更大。在此期间，两组均观察到肝脏胶原蛋白增加，急性期组在5周时达到峰值。该模型有助于研究曼氏血吸虫卵对肉芽肿形成的影响。
